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Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molec...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4762129/ https://www.ncbi.nlm.nih.gov/pubmed/26942220 http://dx.doi.org/10.1007/s41048-015-0002-3 |
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author | Xue, Xiangdong Zhao, Yuanyuan Zhang, Xu Zhang, Chunqiu Kumar, Anil Zhang, Xiaoning Zou, Guozhang Wang, Paul C. Zhang, Jinchao Liang, Xing-Jie |
author_facet | Xue, Xiangdong Zhao, Yuanyuan Zhang, Xu Zhang, Chunqiu Kumar, Anil Zhang, Xiaoning Zou, Guozhang Wang, Paul C. Zhang, Jinchao Liang, Xing-Jie |
author_sort | Xue, Xiangdong |
collection | PubMed |
description | ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different N-glycans were successfully identified from ovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-015-0002-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4762129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47621292016-03-01 Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis Xue, Xiangdong Zhao, Yuanyuan Zhang, Xu Zhang, Chunqiu Kumar, Anil Zhang, Xiaoning Zou, Guozhang Wang, Paul C. Zhang, Jinchao Liang, Xing-Jie Biophys Rep Methods ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different N-glycans were successfully identified from ovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-015-0002-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-07-14 2015 /pmc/articles/PMC4762129/ /pubmed/26942220 http://dx.doi.org/10.1007/s41048-015-0002-3 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Methods Xue, Xiangdong Zhao, Yuanyuan Zhang, Xu Zhang, Chunqiu Kumar, Anil Zhang, Xiaoning Zou, Guozhang Wang, Paul C. Zhang, Jinchao Liang, Xing-Jie Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title | Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title_full | Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title_fullStr | Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title_full_unstemmed | Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title_short | Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
title_sort | phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis |
topic | Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4762129/ https://www.ncbi.nlm.nih.gov/pubmed/26942220 http://dx.doi.org/10.1007/s41048-015-0002-3 |
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