Cargando…

Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis

ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molec...

Descripción completa

Detalles Bibliográficos
Autores principales: Xue, Xiangdong, Zhao, Yuanyuan, Zhang, Xu, Zhang, Chunqiu, Kumar, Anil, Zhang, Xiaoning, Zou, Guozhang, Wang, Paul C., Zhang, Jinchao, Liang, Xing-Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4762129/
https://www.ncbi.nlm.nih.gov/pubmed/26942220
http://dx.doi.org/10.1007/s41048-015-0002-3
_version_ 1782417064656371712
author Xue, Xiangdong
Zhao, Yuanyuan
Zhang, Xu
Zhang, Chunqiu
Kumar, Anil
Zhang, Xiaoning
Zou, Guozhang
Wang, Paul C.
Zhang, Jinchao
Liang, Xing-Jie
author_facet Xue, Xiangdong
Zhao, Yuanyuan
Zhang, Xu
Zhang, Chunqiu
Kumar, Anil
Zhang, Xiaoning
Zou, Guozhang
Wang, Paul C.
Zhang, Jinchao
Liang, Xing-Jie
author_sort Xue, Xiangdong
collection PubMed
description ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different N-glycans were successfully identified from ovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-015-0002-3) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4762129
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-47621292016-03-01 Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis Xue, Xiangdong Zhao, Yuanyuan Zhang, Xu Zhang, Chunqiu Kumar, Anil Zhang, Xiaoning Zou, Guozhang Wang, Paul C. Zhang, Jinchao Liang, Xing-Jie Biophys Rep Methods ABSTRACT: In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different N-glycans were successfully identified from ovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL. GRAPHICAL ABSTRACT: [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s41048-015-0002-3) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-07-14 2015 /pmc/articles/PMC4762129/ /pubmed/26942220 http://dx.doi.org/10.1007/s41048-015-0002-3 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Methods
Xue, Xiangdong
Zhao, Yuanyuan
Zhang, Xu
Zhang, Chunqiu
Kumar, Anil
Zhang, Xiaoning
Zou, Guozhang
Wang, Paul C.
Zhang, Jinchao
Liang, Xing-Jie
Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title_full Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title_fullStr Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title_full_unstemmed Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title_short Phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
title_sort phenylboronic acid-functionalized magnetic nanoparticles for one-step saccharides enrichment and mass spectrometry analysis
topic Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4762129/
https://www.ncbi.nlm.nih.gov/pubmed/26942220
http://dx.doi.org/10.1007/s41048-015-0002-3
work_keys_str_mv AT xuexiangdong phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zhaoyuanyuan phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zhangxu phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zhangchunqiu phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT kumaranil phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zhangxiaoning phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zouguozhang phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT wangpaulc phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT zhangjinchao phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis
AT liangxingjie phenylboronicacidfunctionalizedmagneticnanoparticlesforonestepsaccharidesenrichmentandmassspectrometryanalysis