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Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis

Agarwood, a highly valuable resinous and fragrant heartwood of Aquilaria plants, is widely used in traditional medicines, incense and perfume. Only when Aquilaria trees are wounded by external stimuli do they form agarwood sesquiterpene defensive compounds. Therefore, understanding the signaling pat...

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Autores principales: Xu, Yan-Hong, Liao, Yong-Cui, Zhang, Zheng, Liu, Juan, Sun, Pei-Wen, Gao, Zhi-Hui, Sui, Chun, Wei, Jian-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763180/
https://www.ncbi.nlm.nih.gov/pubmed/26902148
http://dx.doi.org/10.1038/srep21843
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author Xu, Yan-Hong
Liao, Yong-Cui
Zhang, Zheng
Liu, Juan
Sun, Pei-Wen
Gao, Zhi-Hui
Sui, Chun
Wei, Jian-He
author_facet Xu, Yan-Hong
Liao, Yong-Cui
Zhang, Zheng
Liu, Juan
Sun, Pei-Wen
Gao, Zhi-Hui
Sui, Chun
Wei, Jian-He
author_sort Xu, Yan-Hong
collection PubMed
description Agarwood, a highly valuable resinous and fragrant heartwood of Aquilaria plants, is widely used in traditional medicines, incense and perfume. Only when Aquilaria trees are wounded by external stimuli do they form agarwood sesquiterpene defensive compounds. Therefore, understanding the signaling pathway of wound-induced agarwood formation is important. Jasmonic acid (JA) is a well-characterized molecule that mediates a plant’s defense response and secondary metabolism. However, little is known about the function of endogenous JA in agarwood sesquiterpene biosynthesis. Here, we report that heat shock can up-regulate the expression of genes in JA signaling pathway, induce JA production and the accumulation of agarwood sesquiterpene in A. sinensis cell suspension cultures. A specific inhibitor of JA, nordihydroguaiaretic acid (NDGA), could block the JA signaling pathway and reduce the accumulation of sesquiterpene compounds. Additionally, compared to SA and H(2)O(2), exogenously supplied methyl jasmonate has the strongest stimulation effect on the production of sesquiterpene compounds. These results clearly demonstrate the central induction role of JA in heat-shock-induced sesquiterpene production in A. sinensis.
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spelling pubmed-47631802016-03-01 Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis Xu, Yan-Hong Liao, Yong-Cui Zhang, Zheng Liu, Juan Sun, Pei-Wen Gao, Zhi-Hui Sui, Chun Wei, Jian-He Sci Rep Article Agarwood, a highly valuable resinous and fragrant heartwood of Aquilaria plants, is widely used in traditional medicines, incense and perfume. Only when Aquilaria trees are wounded by external stimuli do they form agarwood sesquiterpene defensive compounds. Therefore, understanding the signaling pathway of wound-induced agarwood formation is important. Jasmonic acid (JA) is a well-characterized molecule that mediates a plant’s defense response and secondary metabolism. However, little is known about the function of endogenous JA in agarwood sesquiterpene biosynthesis. Here, we report that heat shock can up-regulate the expression of genes in JA signaling pathway, induce JA production and the accumulation of agarwood sesquiterpene in A. sinensis cell suspension cultures. A specific inhibitor of JA, nordihydroguaiaretic acid (NDGA), could block the JA signaling pathway and reduce the accumulation of sesquiterpene compounds. Additionally, compared to SA and H(2)O(2), exogenously supplied methyl jasmonate has the strongest stimulation effect on the production of sesquiterpene compounds. These results clearly demonstrate the central induction role of JA in heat-shock-induced sesquiterpene production in A. sinensis. Nature Publishing Group 2016-02-23 /pmc/articles/PMC4763180/ /pubmed/26902148 http://dx.doi.org/10.1038/srep21843 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Xu, Yan-Hong
Liao, Yong-Cui
Zhang, Zheng
Liu, Juan
Sun, Pei-Wen
Gao, Zhi-Hui
Sui, Chun
Wei, Jian-He
Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title_full Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title_fullStr Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title_full_unstemmed Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title_short Jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in Aquilaria sinensis
title_sort jasmonic acid is a crucial signal transducer in heat shock induced sesquiterpene formation in aquilaria sinensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763180/
https://www.ncbi.nlm.nih.gov/pubmed/26902148
http://dx.doi.org/10.1038/srep21843
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