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Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions
Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763256/ https://www.ncbi.nlm.nih.gov/pubmed/26902640 http://dx.doi.org/10.1038/srep22073 |
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author | Meng, Yongjie Chen, Feng Shuai, Haiwei Luo, Xiaofeng Ding, Jun Tang, Shengwen Xu, Shuanshuan Liu, Jianwei Liu, Weiguo Du, Junbo Liu, Jiang Yang, Feng Sun, Xin Yong, Taiwen Wang, Xiaochun Feng, Yuqi Shu, Kai Yang, Wenyu |
author_facet | Meng, Yongjie Chen, Feng Shuai, Haiwei Luo, Xiaofeng Ding, Jun Tang, Shengwen Xu, Shuanshuan Liu, Jianwei Liu, Weiguo Du, Junbo Liu, Jiang Yang, Feng Sun, Xin Yong, Taiwen Wang, Xiaochun Feng, Yuqi Shu, Kai Yang, Wenyu |
author_sort | Meng, Yongjie |
collection | PubMed |
description | Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed germination are largely unknown. Here, we report that KAR delayed soybean seed germination through enhancing abscisic acid (ABA) biosynthesis, while impairing gibberellin (GA) biogenesis. Interestingly, KAR only retarded soybean seed germination under shaded conditions, rather than under dark and white light conditions, which differs from in Arabidopsis. Phytohormone quantification showed that KAR enhanced ABA biogenesis while impairing GA biosynthesis during the seed imbibition process, and subsequently, the ratio of active GA(4) to ABA was significantly reduced. Further qRT-PCR analysis showed that the transcription pattern of genes involved in ABA and GA metabolic pathways are consistent with the hormonal measurements. Finally, fluridone, an ABA biogenesis inhibitor, remarkably rescued the delayed-germination phenotype of KAR-treatment; and paclobutrazol, a GA biosynthesis inhibitor, inhibited soybean seed germination. Taken together, these evidences suggest that KAR inhibit soybean seed germination by mediating the ratio between GA and ABA biogenesis. |
format | Online Article Text |
id | pubmed-4763256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47632562016-03-01 Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions Meng, Yongjie Chen, Feng Shuai, Haiwei Luo, Xiaofeng Ding, Jun Tang, Shengwen Xu, Shuanshuan Liu, Jianwei Liu, Weiguo Du, Junbo Liu, Jiang Yang, Feng Sun, Xin Yong, Taiwen Wang, Xiaochun Feng, Yuqi Shu, Kai Yang, Wenyu Sci Rep Article Karrikins (KAR) are a class of signal compounds, discovered in wildfire smoke, which affect seed germination. Currently, numerous studies have focused on the model plant Arabidopsis in the KAR research field, rather than on crops. Thus the regulatory mechanisms underlying KAR regulation of crop seed germination are largely unknown. Here, we report that KAR delayed soybean seed germination through enhancing abscisic acid (ABA) biosynthesis, while impairing gibberellin (GA) biogenesis. Interestingly, KAR only retarded soybean seed germination under shaded conditions, rather than under dark and white light conditions, which differs from in Arabidopsis. Phytohormone quantification showed that KAR enhanced ABA biogenesis while impairing GA biosynthesis during the seed imbibition process, and subsequently, the ratio of active GA(4) to ABA was significantly reduced. Further qRT-PCR analysis showed that the transcription pattern of genes involved in ABA and GA metabolic pathways are consistent with the hormonal measurements. Finally, fluridone, an ABA biogenesis inhibitor, remarkably rescued the delayed-germination phenotype of KAR-treatment; and paclobutrazol, a GA biosynthesis inhibitor, inhibited soybean seed germination. Taken together, these evidences suggest that KAR inhibit soybean seed germination by mediating the ratio between GA and ABA biogenesis. Nature Publishing Group 2016-02-23 /pmc/articles/PMC4763256/ /pubmed/26902640 http://dx.doi.org/10.1038/srep22073 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Meng, Yongjie Chen, Feng Shuai, Haiwei Luo, Xiaofeng Ding, Jun Tang, Shengwen Xu, Shuanshuan Liu, Jianwei Liu, Weiguo Du, Junbo Liu, Jiang Yang, Feng Sun, Xin Yong, Taiwen Wang, Xiaochun Feng, Yuqi Shu, Kai Yang, Wenyu Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title | Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title_full | Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title_fullStr | Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title_full_unstemmed | Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title_short | Karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
title_sort | karrikins delay soybean seed germination by mediating abscisic acid and gibberellin biogenesis under shaded conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763256/ https://www.ncbi.nlm.nih.gov/pubmed/26902640 http://dx.doi.org/10.1038/srep22073 |
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