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Expression profiles of sugarcane under drought conditions: Variation in gene regulation
Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of change...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763319/ https://www.ncbi.nlm.nih.gov/pubmed/26537606 http://dx.doi.org/10.1590/S1415-475738420140288 |
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author | de Andrade, Júlio César Farias Terto, Jackeline Silva, José Vieira Almeida, Cícero |
author_facet | de Andrade, Júlio César Farias Terto, Jackeline Silva, José Vieira Almeida, Cícero |
author_sort | de Andrade, Júlio César Farias |
collection | PubMed |
description | Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (g (s)) and stomatal transpiration (E) were measured. Total RNA was extracted from leaves and the expression of SAMDC, ZmPIP2-1 protein, ZmTIP4-2 protein, WIP protein, LTP protein, histone H3, DNAj, ferredoxin I, β-tubulin, photosystem I, gene 1 and gene 2 was analyzed by quantitative real-time PCR (RT-PCR). Important differences in the expression profiles of these genes were observed when compared with other genotypes, suggesting that complex defense mechanisms are activated in response to water stress. However, there was no recognizable pattern for the changes in expression of the different proteins associated with tolerance to drought stress. |
format | Online Article Text |
id | pubmed-4763319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-47633192016-03-08 Expression profiles of sugarcane under drought conditions: Variation in gene regulation de Andrade, Júlio César Farias Terto, Jackeline Silva, José Vieira Almeida, Cícero Genet Mol Biol Short Communication Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (g (s)) and stomatal transpiration (E) were measured. Total RNA was extracted from leaves and the expression of SAMDC, ZmPIP2-1 protein, ZmTIP4-2 protein, WIP protein, LTP protein, histone H3, DNAj, ferredoxin I, β-tubulin, photosystem I, gene 1 and gene 2 was analyzed by quantitative real-time PCR (RT-PCR). Important differences in the expression profiles of these genes were observed when compared with other genotypes, suggesting that complex defense mechanisms are activated in response to water stress. However, there was no recognizable pattern for the changes in expression of the different proteins associated with tolerance to drought stress. Sociedade Brasileira de Genética 2015 /pmc/articles/PMC4763319/ /pubmed/26537606 http://dx.doi.org/10.1590/S1415-475738420140288 Text en Copyright © 2015, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. |
spellingShingle | Short Communication de Andrade, Júlio César Farias Terto, Jackeline Silva, José Vieira Almeida, Cícero Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title | Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title_full | Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title_fullStr | Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title_full_unstemmed | Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title_short | Expression profiles of sugarcane under drought conditions: Variation in gene regulation |
title_sort | expression profiles of sugarcane under drought conditions: variation in gene regulation |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763319/ https://www.ncbi.nlm.nih.gov/pubmed/26537606 http://dx.doi.org/10.1590/S1415-475738420140288 |
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