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Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis

Granulomatosis with polyangiitis is a rare chronic inflammatory disease. In this multisystem autoimmune disorder neutrophils cause small vessels necrosis and infiltrate perivascular tissue to form granulomas. Progression of the disease is evaluated by the symptoms score and by a titer of anti-neutro...

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Autores principales: Surmiak, Marcin, Hubalewska-Mazgaj, Magdalena, Wawrzycka-Adamczyk, Katarzyna, Musiał, Jacek, Sanak, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763974/
https://www.ncbi.nlm.nih.gov/pubmed/27047256
http://dx.doi.org/10.2174/1389202916666150707160434
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author Surmiak, Marcin
Hubalewska-Mazgaj, Magdalena
Wawrzycka-Adamczyk, Katarzyna
Musiał, Jacek
Sanak, Marek
author_facet Surmiak, Marcin
Hubalewska-Mazgaj, Magdalena
Wawrzycka-Adamczyk, Katarzyna
Musiał, Jacek
Sanak, Marek
author_sort Surmiak, Marcin
collection PubMed
description Granulomatosis with polyangiitis is a rare chronic inflammatory disease. In this multisystem autoimmune disorder neutrophils cause small vessels necrosis and infiltrate perivascular tissue to form granulomas. Progression of the disease is evaluated by the symptoms score and by a titer of anti-neutrophil cytoplasm antibodies. Despite glucocorticoid and immunosuppressive therapy, prognosis is complicated by chronic renal insufficiency, hearing loss and skin ulceration. In this preliminary study we tested the hypothesis that altered neutrophil expression of miRNAs can contribute to the cell activation, extracellular traps formation and decreased apoptosis. First we compared a profile of 728 miRNAs expressed in circulating neutrophils of patients with active disease and matched healthy donors. Subsequently, candidate miRNAs were quantified in neutrophils from 16 subjects with active disease, 16 asymptomatic patients at the remission and in 16 healthy controls. Out of 11 candidate miRNAs, only miR-128-3p was both biologically (relative quantity < 30% control or remission patients) and statistically (p<0.01) decreased in the cells during active stage of the disease. This miRNA correlated with a clinical score of the disease well. A set of 10 transcripts involved in the mechanism of the disease was quantified from the same neutrophils RNA. Relative expression of MMP9 was higher in neutrophils from the patients with active disease and correlated negatively with miR-128-3p. The opposite finding was present for MTA1 transcripts. Despite surprisingly scarce changes in the expression of neutrophil miRNAs, miR-128-3p is the best candidate for deciphering etiology of granulomatosis with polyangiitis.
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spelling pubmed-47639742016-04-04 Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis Surmiak, Marcin Hubalewska-Mazgaj, Magdalena Wawrzycka-Adamczyk, Katarzyna Musiał, Jacek Sanak, Marek Curr Genomics Article Granulomatosis with polyangiitis is a rare chronic inflammatory disease. In this multisystem autoimmune disorder neutrophils cause small vessels necrosis and infiltrate perivascular tissue to form granulomas. Progression of the disease is evaluated by the symptoms score and by a titer of anti-neutrophil cytoplasm antibodies. Despite glucocorticoid and immunosuppressive therapy, prognosis is complicated by chronic renal insufficiency, hearing loss and skin ulceration. In this preliminary study we tested the hypothesis that altered neutrophil expression of miRNAs can contribute to the cell activation, extracellular traps formation and decreased apoptosis. First we compared a profile of 728 miRNAs expressed in circulating neutrophils of patients with active disease and matched healthy donors. Subsequently, candidate miRNAs were quantified in neutrophils from 16 subjects with active disease, 16 asymptomatic patients at the remission and in 16 healthy controls. Out of 11 candidate miRNAs, only miR-128-3p was both biologically (relative quantity < 30% control or remission patients) and statistically (p<0.01) decreased in the cells during active stage of the disease. This miRNA correlated with a clinical score of the disease well. A set of 10 transcripts involved in the mechanism of the disease was quantified from the same neutrophils RNA. Relative expression of MMP9 was higher in neutrophils from the patients with active disease and correlated negatively with miR-128-3p. The opposite finding was present for MTA1 transcripts. Despite surprisingly scarce changes in the expression of neutrophil miRNAs, miR-128-3p is the best candidate for deciphering etiology of granulomatosis with polyangiitis. Bentham Science Publishers 2015-10 2015-10 /pmc/articles/PMC4763974/ /pubmed/27047256 http://dx.doi.org/10.2174/1389202916666150707160434 Text en ©2015 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Surmiak, Marcin
Hubalewska-Mazgaj, Magdalena
Wawrzycka-Adamczyk, Katarzyna
Musiał, Jacek
Sanak, Marek
Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title_full Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title_fullStr Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title_full_unstemmed Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title_short Neutrophil MiRNA-128-3p is Decreased During Active Phase of Granulo-matosis with Polyangiitis
title_sort neutrophil mirna-128-3p is decreased during active phase of granulo-matosis with polyangiitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763974/
https://www.ncbi.nlm.nih.gov/pubmed/27047256
http://dx.doi.org/10.2174/1389202916666150707160434
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