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Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice

OBJECTIVE(S): Both caloric restriction (CR) and resveratrol (RSV) have been shown to improve learning and memory, but their potential effects in juvenile animals were unknown. Here, we evaluated the effects of RSV and CR on learning and memory function in juvenile mice and investigated potential mol...

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Autores principales: Xu, Bao-Lei, Wang, Rong, Ma, Li-Na, Dong, Wen, Zhao, Zhi-Wei, Zhang, Jing-Shuang, Wang, Yu-Lan, Zhang, Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764114/
https://www.ncbi.nlm.nih.gov/pubmed/26949500
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author Xu, Bao-Lei
Wang, Rong
Ma, Li-Na
Dong, Wen
Zhao, Zhi-Wei
Zhang, Jing-Shuang
Wang, Yu-Lan
Zhang, Xu
author_facet Xu, Bao-Lei
Wang, Rong
Ma, Li-Na
Dong, Wen
Zhao, Zhi-Wei
Zhang, Jing-Shuang
Wang, Yu-Lan
Zhang, Xu
author_sort Xu, Bao-Lei
collection PubMed
description OBJECTIVE(S): Both caloric restriction (CR) and resveratrol (RSV) have been shown to improve learning and memory, but their potential effects in juvenile animals were unknown. Here, we evaluated the effects of RSV and CR on learning and memory function in juvenile mice and investigated potential molecular mechanisms. METHODS: Six-week-old C57BL/6J mice were assigned to one of three different dietary groups: normal control (stock diet) (n=12), CR diet (30% caloric reduction diet) (n=12), and RSV diet (stock diet supplemented with 18.6 mg/kg RSV) (n=12), for 6 months. Body weight and blood glucose were measured every 4 weeks. Serum cholesterol and serum triglyceride levels were examined using biochemical methods. Serum insulin and insulin-like growth factor 1 (IGF-1) levels were evaluated using enzyme linked immunosorbant assay (ELISA), and protein expression of silent mating type information regulation 2 homology 1 (SIRT1), p53, p16, peroxisome proliferator-activated receptor γ (PPARγ), phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K), phosphorylated-cAMP response element-binding protein (p-CREB), and IGF-1 were examined with immunohistochemistry. RESULTS: Although long-term CR diet did not alter physiological conditions in juvenile mice relative to control, RSV supplementation slightly elevated blood glucose, serum triglyceride, and serum insulin levels. Both CR and RSV improved learning and memory function, although the effect of CR was significantly greater. Both CR and RSV downregulated p53 and upregulated IGF-1 in hippocampal CA1 region of mice. CONCLUSION: We demonstrate that CR and RSV may improve learning and memory by downregulating p53 and upregulating IGF-1 in hippocampal CA1 region of juvenile mice.
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spelling pubmed-47641142016-03-04 Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice Xu, Bao-Lei Wang, Rong Ma, Li-Na Dong, Wen Zhao, Zhi-Wei Zhang, Jing-Shuang Wang, Yu-Lan Zhang, Xu Iran J Basic Med Sci Original Article OBJECTIVE(S): Both caloric restriction (CR) and resveratrol (RSV) have been shown to improve learning and memory, but their potential effects in juvenile animals were unknown. Here, we evaluated the effects of RSV and CR on learning and memory function in juvenile mice and investigated potential molecular mechanisms. METHODS: Six-week-old C57BL/6J mice were assigned to one of three different dietary groups: normal control (stock diet) (n=12), CR diet (30% caloric reduction diet) (n=12), and RSV diet (stock diet supplemented with 18.6 mg/kg RSV) (n=12), for 6 months. Body weight and blood glucose were measured every 4 weeks. Serum cholesterol and serum triglyceride levels were examined using biochemical methods. Serum insulin and insulin-like growth factor 1 (IGF-1) levels were evaluated using enzyme linked immunosorbant assay (ELISA), and protein expression of silent mating type information regulation 2 homology 1 (SIRT1), p53, p16, peroxisome proliferator-activated receptor γ (PPARγ), phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K), phosphorylated-cAMP response element-binding protein (p-CREB), and IGF-1 were examined with immunohistochemistry. RESULTS: Although long-term CR diet did not alter physiological conditions in juvenile mice relative to control, RSV supplementation slightly elevated blood glucose, serum triglyceride, and serum insulin levels. Both CR and RSV improved learning and memory function, although the effect of CR was significantly greater. Both CR and RSV downregulated p53 and upregulated IGF-1 in hippocampal CA1 region of mice. CONCLUSION: We demonstrate that CR and RSV may improve learning and memory by downregulating p53 and upregulating IGF-1 in hippocampal CA1 region of juvenile mice. Mashhad University of Medical Sciences 2015-11 /pmc/articles/PMC4764114/ /pubmed/26949500 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Xu, Bao-Lei
Wang, Rong
Ma, Li-Na
Dong, Wen
Zhao, Zhi-Wei
Zhang, Jing-Shuang
Wang, Yu-Lan
Zhang, Xu
Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title_full Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title_fullStr Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title_full_unstemmed Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title_short Comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile C57BL/6J mice
title_sort comparison of the effects of resveratrol and caloric restriction on learning and memory in juvenile c57bl/6j mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764114/
https://www.ncbi.nlm.nih.gov/pubmed/26949500
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