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A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children

BACKGROUND: Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY: Among 63 confirmed dengue pediatric patients recru...

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Autores principales: Nhi, Dang My, Huy, Nguyen Tien, Ohyama, Kaname, Kimura, Daisuke, Lan, Nguyen Thi Phuong, Uchida, Leo, Thuong, Nguyen Van, Nhon, Cao Thi My, Phuc, Le Hong, Mai, Nguyen Thi, Mizukami, Shusaku, Bao, Lam Quoc, Doan, Nguyen Ngoc, Binh, Nguyen Van Thanh, Quang, Luong Chan, Karbwang, Juntra, Yui, Katsuyuki, Morita, Kouichi, Huong, Vu Thi Que, Hirayama, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764501/
https://www.ncbi.nlm.nih.gov/pubmed/26895439
http://dx.doi.org/10.1371/journal.pntd.0004435
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author Nhi, Dang My
Huy, Nguyen Tien
Ohyama, Kaname
Kimura, Daisuke
Lan, Nguyen Thi Phuong
Uchida, Leo
Thuong, Nguyen Van
Nhon, Cao Thi My
Phuc, Le Hong
Mai, Nguyen Thi
Mizukami, Shusaku
Bao, Lam Quoc
Doan, Nguyen Ngoc
Binh, Nguyen Van Thanh
Quang, Luong Chan
Karbwang, Juntra
Yui, Katsuyuki
Morita, Kouichi
Huong, Vu Thi Que
Hirayama, Kenji
author_facet Nhi, Dang My
Huy, Nguyen Tien
Ohyama, Kaname
Kimura, Daisuke
Lan, Nguyen Thi Phuong
Uchida, Leo
Thuong, Nguyen Van
Nhon, Cao Thi My
Phuc, Le Hong
Mai, Nguyen Thi
Mizukami, Shusaku
Bao, Lam Quoc
Doan, Nguyen Ngoc
Binh, Nguyen Van Thanh
Quang, Luong Chan
Karbwang, Juntra
Yui, Katsuyuki
Morita, Kouichi
Huong, Vu Thi Que
Hirayama, Kenji
author_sort Nhi, Dang My
collection PubMed
description BACKGROUND: Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY: Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6–48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group. Validation was undertaken in 6 SD-SPL and 10 DWS patients. PRINCIPAL FINDINGS: Nineteen plasma proteins exhibited significantly different relative concentrations (p<0.05), with five over-expressed and fourteen under-expressed in SD-SPL compared with DWS. The individual protein was classified to either blood coagulation, vascular regulation, cellular transport-related processes or immune response. The immunoblot quantification showed angiotensinogen and antithrombin III significantly increased in SD-SPL whole plasma of early stage compared with DWS subjects. Even using this small number of samples, antithrombin III predicted SD-SPL before shock occurrence with accuracy. CONCLUSION: Proteins identified here may serve as candidate predictive markers to diagnose SD-SPL for timely clinical management. Since the number of subjects are small, so further studies are needed to confirm all these biomarkers.
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spelling pubmed-47645012016-03-07 A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children Nhi, Dang My Huy, Nguyen Tien Ohyama, Kaname Kimura, Daisuke Lan, Nguyen Thi Phuong Uchida, Leo Thuong, Nguyen Van Nhon, Cao Thi My Phuc, Le Hong Mai, Nguyen Thi Mizukami, Shusaku Bao, Lam Quoc Doan, Nguyen Ngoc Binh, Nguyen Van Thanh Quang, Luong Chan Karbwang, Juntra Yui, Katsuyuki Morita, Kouichi Huong, Vu Thi Que Hirayama, Kenji PLoS Negl Trop Dis Research Article BACKGROUND: Severe dengue with severe plasma leakage (SD-SPL) is the most frequent of dengue severe form. Plasma biomarkers for early predictive diagnosis of SD-SPL are required in the primary clinics for the prevention of dengue death. METHODOLOGY: Among 63 confirmed dengue pediatric patients recruited, hospital based longitudinal study detected six SD-SPL and ten dengue with warning sign (DWS). To identify the specific proteins increased or decreased in the SD-SPL plasma obtained 6–48 hours before the shock compared with the DWS, the isobaric tags for relative and absolute quantification (iTRAQ) technology was performed using four patients each group. Validation was undertaken in 6 SD-SPL and 10 DWS patients. PRINCIPAL FINDINGS: Nineteen plasma proteins exhibited significantly different relative concentrations (p<0.05), with five over-expressed and fourteen under-expressed in SD-SPL compared with DWS. The individual protein was classified to either blood coagulation, vascular regulation, cellular transport-related processes or immune response. The immunoblot quantification showed angiotensinogen and antithrombin III significantly increased in SD-SPL whole plasma of early stage compared with DWS subjects. Even using this small number of samples, antithrombin III predicted SD-SPL before shock occurrence with accuracy. CONCLUSION: Proteins identified here may serve as candidate predictive markers to diagnose SD-SPL for timely clinical management. Since the number of subjects are small, so further studies are needed to confirm all these biomarkers. Public Library of Science 2016-02-19 /pmc/articles/PMC4764501/ /pubmed/26895439 http://dx.doi.org/10.1371/journal.pntd.0004435 Text en © 2016 Nhi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nhi, Dang My
Huy, Nguyen Tien
Ohyama, Kaname
Kimura, Daisuke
Lan, Nguyen Thi Phuong
Uchida, Leo
Thuong, Nguyen Van
Nhon, Cao Thi My
Phuc, Le Hong
Mai, Nguyen Thi
Mizukami, Shusaku
Bao, Lam Quoc
Doan, Nguyen Ngoc
Binh, Nguyen Van Thanh
Quang, Luong Chan
Karbwang, Juntra
Yui, Katsuyuki
Morita, Kouichi
Huong, Vu Thi Que
Hirayama, Kenji
A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title_full A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title_fullStr A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title_full_unstemmed A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title_short A Proteomic Approach Identifies Candidate Early Biomarkers to Predict Severe Dengue in Children
title_sort proteomic approach identifies candidate early biomarkers to predict severe dengue in children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764501/
https://www.ncbi.nlm.nih.gov/pubmed/26895439
http://dx.doi.org/10.1371/journal.pntd.0004435
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