Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome

Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that the specific interaction betwee...

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Autores principales: Fang, Qianglin, Chen, Ping, Wang, Mingzhu, Fang, Junnan, Yang, Na, Li, Guohong, Xu, Rui-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764553/
https://www.ncbi.nlm.nih.gov/pubmed/26812545
http://dx.doi.org/10.7554/eLife.11911
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author Fang, Qianglin
Chen, Ping
Wang, Mingzhu
Fang, Junnan
Yang, Na
Li, Guohong
Xu, Rui-Ming
author_facet Fang, Qianglin
Chen, Ping
Wang, Mingzhu
Fang, Junnan
Yang, Na
Li, Guohong
Xu, Rui-Ming
author_sort Fang, Qianglin
collection PubMed
description Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that the specific interaction between IE1-CTD and the H2A-H2B acidic patch impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections. DOI: http://dx.doi.org/10.7554/eLife.11911.001
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spelling pubmed-47645532016-02-25 Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome Fang, Qianglin Chen, Ping Wang, Mingzhu Fang, Junnan Yang, Na Li, Guohong Xu, Rui-Ming eLife Biochemistry Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that the specific interaction between IE1-CTD and the H2A-H2B acidic patch impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections. DOI: http://dx.doi.org/10.7554/eLife.11911.001 eLife Sciences Publications, Ltd 2016-01-26 /pmc/articles/PMC4764553/ /pubmed/26812545 http://dx.doi.org/10.7554/eLife.11911 Text en © 2016, Fang et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biochemistry
Fang, Qianglin
Chen, Ping
Wang, Mingzhu
Fang, Junnan
Yang, Na
Li, Guohong
Xu, Rui-Ming
Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title_full Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title_fullStr Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title_full_unstemmed Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title_short Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
title_sort human cytomegalovirus ie1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764553/
https://www.ncbi.nlm.nih.gov/pubmed/26812545
http://dx.doi.org/10.7554/eLife.11911
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