A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea

The extracellular matrix (ECM), a structure contributed to and commonly shared by many cells in an organism, plays an active role during morphogenesis. Here, we used the Drosophila tracheal system to study the complex relationship between the ECM and epithelial cells during development. We show that...

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Autores principales: Öztürk-Çolak, Arzu, Moussian, Bernard, Araújo, Sofia J, Casanova, Jordi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764556/
https://www.ncbi.nlm.nih.gov/pubmed/26836303
http://dx.doi.org/10.7554/eLife.09373
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author Öztürk-Çolak, Arzu
Moussian, Bernard
Araújo, Sofia J
Casanova, Jordi
author_facet Öztürk-Çolak, Arzu
Moussian, Bernard
Araújo, Sofia J
Casanova, Jordi
author_sort Öztürk-Çolak, Arzu
collection PubMed
description The extracellular matrix (ECM), a structure contributed to and commonly shared by many cells in an organism, plays an active role during morphogenesis. Here, we used the Drosophila tracheal system to study the complex relationship between the ECM and epithelial cells during development. We show that there is an active feedback mechanism between the apical ECM (aECM) and the apical F-actin in tracheal cells. Furthermore, we reveal that cell-cell junctions are key players in this aECM patterning and organisation and that individual cells contribute autonomously to their aECM. Strikingly, changes in the aECM influence the levels of phosphorylated Src42A (pSrc) at cell junctions. Therefore, we propose that Src42A phosphorylation levels provide a link for the ECM environment to ensure proper cytoskeletal organisation. DOI: http://dx.doi.org/10.7554/eLife.09373.001
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spelling pubmed-47645562016-02-25 A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea Öztürk-Çolak, Arzu Moussian, Bernard Araújo, Sofia J Casanova, Jordi eLife Cell Biology The extracellular matrix (ECM), a structure contributed to and commonly shared by many cells in an organism, plays an active role during morphogenesis. Here, we used the Drosophila tracheal system to study the complex relationship between the ECM and epithelial cells during development. We show that there is an active feedback mechanism between the apical ECM (aECM) and the apical F-actin in tracheal cells. Furthermore, we reveal that cell-cell junctions are key players in this aECM patterning and organisation and that individual cells contribute autonomously to their aECM. Strikingly, changes in the aECM influence the levels of phosphorylated Src42A (pSrc) at cell junctions. Therefore, we propose that Src42A phosphorylation levels provide a link for the ECM environment to ensure proper cytoskeletal organisation. DOI: http://dx.doi.org/10.7554/eLife.09373.001 eLife Sciences Publications, Ltd 2016-02-02 /pmc/articles/PMC4764556/ /pubmed/26836303 http://dx.doi.org/10.7554/eLife.09373 Text en © 2016, Öztürk-Çolak et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Öztürk-Çolak, Arzu
Moussian, Bernard
Araújo, Sofia J
Casanova, Jordi
A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title_full A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title_fullStr A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title_full_unstemmed A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title_short A feedback mechanism converts individual cell features into a supracellular ECM structure in Drosophila trachea
title_sort feedback mechanism converts individual cell features into a supracellular ecm structure in drosophila trachea
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764556/
https://www.ncbi.nlm.nih.gov/pubmed/26836303
http://dx.doi.org/10.7554/eLife.09373
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