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Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins
AIM: We determined the role of brain Gαi(2) proteins in mediating the neural and humoral responses of conscious male Sprague–Dawley rats to acute peripheral sodium challenge. METHODS: Rats pre‐treated (24‐h) intracerebroventricularly with a targeted oligodeoxynucleotide (ODN) (25 μg per 5 μL) to dow...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764872/ https://www.ncbi.nlm.nih.gov/pubmed/26412230 http://dx.doi.org/10.1111/apha.12610 |
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author | Carmichael, C. Y. Carmichael, A. C. T. Kuwabara, J. T. Cunningham, J. T. Wainford, R. D. |
author_facet | Carmichael, C. Y. Carmichael, A. C. T. Kuwabara, J. T. Cunningham, J. T. Wainford, R. D. |
author_sort | Carmichael, C. Y. |
collection | PubMed |
description | AIM: We determined the role of brain Gαi(2) proteins in mediating the neural and humoral responses of conscious male Sprague–Dawley rats to acute peripheral sodium challenge. METHODS: Rats pre‐treated (24‐h) intracerebroventricularly with a targeted oligodeoxynucleotide (ODN) (25 μg per 5 μL) to downregulate brain Gαi(2) protein expression or a scrambled (SCR) control ODN were challenged with an acute sodium load (intravenous bolus 3 m NaCl; 0.14 mL per 100 g), and cardiovascular parameters were monitored for 120 min. In additional groups, hypothalamic paraventricular nucleus (PVN) Fos immunoreactivity was examined at baseline, 40, and 100 min post‐sodium challenge. RESULTS: In response to intravenous hypertonic saline (HS), no difference was observed in peak change in mean arterial pressure between groups. In SCR ODN pre‐treated rats, arterial pressure returned to baseline by 100 min, while it remained elevated in Gαi(2) ODN pre‐treated rats (P < 0.05). No difference between groups was observed in sodium‐evoked increases in Fos‐positive magnocellular neurons or vasopressin release. V(1a) receptor antagonism failed to block the prolonged elevation of arterial pressure in Gαi(2) ODN pre‐treated rats. A significantly greater number of Fos‐positive ventrolateral parvocellular, lateral parvocellular, and medial parvocellular neurons were observed in SCR vs. Gαi(2) ODN pre‐treated rats at 40 and 100 min post‐HS challenge (P < 0.05). In SCR, but not Gαi(2) ODN pre‐treated rats, HS evoked suppression of plasma norepinephrine (P < 0.05). CONCLUSION: This highlights Gαi(2) protein signal transduction as a novel central mechanism acting to differentially influence PVN parvocellular neuronal activation, sympathetic outflow, and arterial pressure in response to acute HS, independently of actions on magnocellular neurons and vasopressin release. |
format | Online Article Text |
id | pubmed-4764872 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47648722016-03-01 Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins Carmichael, C. Y. Carmichael, A. C. T. Kuwabara, J. T. Cunningham, J. T. Wainford, R. D. Acta Physiol (Oxf) Original Articles AIM: We determined the role of brain Gαi(2) proteins in mediating the neural and humoral responses of conscious male Sprague–Dawley rats to acute peripheral sodium challenge. METHODS: Rats pre‐treated (24‐h) intracerebroventricularly with a targeted oligodeoxynucleotide (ODN) (25 μg per 5 μL) to downregulate brain Gαi(2) protein expression or a scrambled (SCR) control ODN were challenged with an acute sodium load (intravenous bolus 3 m NaCl; 0.14 mL per 100 g), and cardiovascular parameters were monitored for 120 min. In additional groups, hypothalamic paraventricular nucleus (PVN) Fos immunoreactivity was examined at baseline, 40, and 100 min post‐sodium challenge. RESULTS: In response to intravenous hypertonic saline (HS), no difference was observed in peak change in mean arterial pressure between groups. In SCR ODN pre‐treated rats, arterial pressure returned to baseline by 100 min, while it remained elevated in Gαi(2) ODN pre‐treated rats (P < 0.05). No difference between groups was observed in sodium‐evoked increases in Fos‐positive magnocellular neurons or vasopressin release. V(1a) receptor antagonism failed to block the prolonged elevation of arterial pressure in Gαi(2) ODN pre‐treated rats. A significantly greater number of Fos‐positive ventrolateral parvocellular, lateral parvocellular, and medial parvocellular neurons were observed in SCR vs. Gαi(2) ODN pre‐treated rats at 40 and 100 min post‐HS challenge (P < 0.05). In SCR, but not Gαi(2) ODN pre‐treated rats, HS evoked suppression of plasma norepinephrine (P < 0.05). CONCLUSION: This highlights Gαi(2) protein signal transduction as a novel central mechanism acting to differentially influence PVN parvocellular neuronal activation, sympathetic outflow, and arterial pressure in response to acute HS, independently of actions on magnocellular neurons and vasopressin release. John Wiley and Sons Inc. 2016-03 2015-10-19 /pmc/articles/PMC4764872/ /pubmed/26412230 http://dx.doi.org/10.1111/apha.12610 Text en © 2015 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Carmichael, C. Y. Carmichael, A. C. T. Kuwabara, J. T. Cunningham, J. T. Wainford, R. D. Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title | Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title_full | Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title_fullStr | Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title_full_unstemmed | Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title_short | Impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious Sprague–Dawley rats lacking central Gαi(2) proteins |
title_sort | impaired sodium‐evoked paraventricular nucleus neuronal activation and blood pressure regulation in conscious sprague–dawley rats lacking central gαi(2) proteins |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764872/ https://www.ncbi.nlm.nih.gov/pubmed/26412230 http://dx.doi.org/10.1111/apha.12610 |
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