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Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts

Pneumocystis jirovecii is a major cause of life-threatening pneumonia in immunosuppressed patients including transplant recipients and those with HIV/AIDS, yet surprisingly little is known about the biology of this fungal pathogen. Here we report near complete genome assemblies for three Pneumocysti...

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Autores principales: Ma, Liang, Chen, Zehua, Huang, Da Wei, Kutty, Geetha, Ishihara, Mayumi, Wang, Honghui, Abouelleil, Amr, Bishop, Lisa, Davey, Emma, Deng, Rebecca, Deng, Xilong, Fan, Lin, Fantoni, Giovanna, Fitzgerald, Michael, Gogineni, Emile, Goldberg, Jonathan M., Handley, Grace, Hu, Xiaojun, Huber, Charles, Jiao, Xiaoli, Jones, Kristine, Levin, Joshua Z., Liu, Yueqin, Macdonald, Pendexter, Melnikov, Alexandre, Raley, Castle, Sassi, Monica, Sherman, Brad T., Song, Xiaohong, Sykes, Sean, Tran, Bao, Walsh, Laura, Xia, Yun, Yang, Jun, Young, Sarah, Zeng, Qiandong, Zheng, Xin, Stephens, Robert, Nusbaum, Chad, Birren, Bruce W., Azadi, Parastoo, Lempicki, Richard A., Cuomo, Christina A., Kovacs, Joseph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764891/
https://www.ncbi.nlm.nih.gov/pubmed/26899007
http://dx.doi.org/10.1038/ncomms10740
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author Ma, Liang
Chen, Zehua
Huang, Da Wei
Kutty, Geetha
Ishihara, Mayumi
Wang, Honghui
Abouelleil, Amr
Bishop, Lisa
Davey, Emma
Deng, Rebecca
Deng, Xilong
Fan, Lin
Fantoni, Giovanna
Fitzgerald, Michael
Gogineni, Emile
Goldberg, Jonathan M.
Handley, Grace
Hu, Xiaojun
Huber, Charles
Jiao, Xiaoli
Jones, Kristine
Levin, Joshua Z.
Liu, Yueqin
Macdonald, Pendexter
Melnikov, Alexandre
Raley, Castle
Sassi, Monica
Sherman, Brad T.
Song, Xiaohong
Sykes, Sean
Tran, Bao
Walsh, Laura
Xia, Yun
Yang, Jun
Young, Sarah
Zeng, Qiandong
Zheng, Xin
Stephens, Robert
Nusbaum, Chad
Birren, Bruce W.
Azadi, Parastoo
Lempicki, Richard A.
Cuomo, Christina A.
Kovacs, Joseph A.
author_facet Ma, Liang
Chen, Zehua
Huang, Da Wei
Kutty, Geetha
Ishihara, Mayumi
Wang, Honghui
Abouelleil, Amr
Bishop, Lisa
Davey, Emma
Deng, Rebecca
Deng, Xilong
Fan, Lin
Fantoni, Giovanna
Fitzgerald, Michael
Gogineni, Emile
Goldberg, Jonathan M.
Handley, Grace
Hu, Xiaojun
Huber, Charles
Jiao, Xiaoli
Jones, Kristine
Levin, Joshua Z.
Liu, Yueqin
Macdonald, Pendexter
Melnikov, Alexandre
Raley, Castle
Sassi, Monica
Sherman, Brad T.
Song, Xiaohong
Sykes, Sean
Tran, Bao
Walsh, Laura
Xia, Yun
Yang, Jun
Young, Sarah
Zeng, Qiandong
Zheng, Xin
Stephens, Robert
Nusbaum, Chad
Birren, Bruce W.
Azadi, Parastoo
Lempicki, Richard A.
Cuomo, Christina A.
Kovacs, Joseph A.
author_sort Ma, Liang
collection PubMed
description Pneumocystis jirovecii is a major cause of life-threatening pneumonia in immunosuppressed patients including transplant recipients and those with HIV/AIDS, yet surprisingly little is known about the biology of this fungal pathogen. Here we report near complete genome assemblies for three Pneumocystis species that infect humans, rats and mice. Pneumocystis genomes are highly compact relative to other fungi, with substantial reductions of ribosomal RNA genes, transporters, transcription factors and many metabolic pathways, but contain expansions of surface proteins, especially a unique and complex surface glycoprotein superfamily, as well as proteases and RNA processing proteins. Unexpectedly, the key fungal cell wall components chitin and outer chain N-mannans are absent, based on genome content and experimental validation. Our findings suggest that Pneumocystis has developed unique mechanisms of adaptation to life exclusively in mammalian hosts, including dependence on the lungs for gas and nutrients and highly efficient strategies to escape both host innate and acquired immune defenses.
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spelling pubmed-47648912016-03-04 Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts Ma, Liang Chen, Zehua Huang, Da Wei Kutty, Geetha Ishihara, Mayumi Wang, Honghui Abouelleil, Amr Bishop, Lisa Davey, Emma Deng, Rebecca Deng, Xilong Fan, Lin Fantoni, Giovanna Fitzgerald, Michael Gogineni, Emile Goldberg, Jonathan M. Handley, Grace Hu, Xiaojun Huber, Charles Jiao, Xiaoli Jones, Kristine Levin, Joshua Z. Liu, Yueqin Macdonald, Pendexter Melnikov, Alexandre Raley, Castle Sassi, Monica Sherman, Brad T. Song, Xiaohong Sykes, Sean Tran, Bao Walsh, Laura Xia, Yun Yang, Jun Young, Sarah Zeng, Qiandong Zheng, Xin Stephens, Robert Nusbaum, Chad Birren, Bruce W. Azadi, Parastoo Lempicki, Richard A. Cuomo, Christina A. Kovacs, Joseph A. Nat Commun Article Pneumocystis jirovecii is a major cause of life-threatening pneumonia in immunosuppressed patients including transplant recipients and those with HIV/AIDS, yet surprisingly little is known about the biology of this fungal pathogen. Here we report near complete genome assemblies for three Pneumocystis species that infect humans, rats and mice. Pneumocystis genomes are highly compact relative to other fungi, with substantial reductions of ribosomal RNA genes, transporters, transcription factors and many metabolic pathways, but contain expansions of surface proteins, especially a unique and complex surface glycoprotein superfamily, as well as proteases and RNA processing proteins. Unexpectedly, the key fungal cell wall components chitin and outer chain N-mannans are absent, based on genome content and experimental validation. Our findings suggest that Pneumocystis has developed unique mechanisms of adaptation to life exclusively in mammalian hosts, including dependence on the lungs for gas and nutrients and highly efficient strategies to escape both host innate and acquired immune defenses. Nature Publishing Group 2016-02-22 /pmc/articles/PMC4764891/ /pubmed/26899007 http://dx.doi.org/10.1038/ncomms10740 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ma, Liang
Chen, Zehua
Huang, Da Wei
Kutty, Geetha
Ishihara, Mayumi
Wang, Honghui
Abouelleil, Amr
Bishop, Lisa
Davey, Emma
Deng, Rebecca
Deng, Xilong
Fan, Lin
Fantoni, Giovanna
Fitzgerald, Michael
Gogineni, Emile
Goldberg, Jonathan M.
Handley, Grace
Hu, Xiaojun
Huber, Charles
Jiao, Xiaoli
Jones, Kristine
Levin, Joshua Z.
Liu, Yueqin
Macdonald, Pendexter
Melnikov, Alexandre
Raley, Castle
Sassi, Monica
Sherman, Brad T.
Song, Xiaohong
Sykes, Sean
Tran, Bao
Walsh, Laura
Xia, Yun
Yang, Jun
Young, Sarah
Zeng, Qiandong
Zheng, Xin
Stephens, Robert
Nusbaum, Chad
Birren, Bruce W.
Azadi, Parastoo
Lempicki, Richard A.
Cuomo, Christina A.
Kovacs, Joseph A.
Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title_full Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title_fullStr Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title_full_unstemmed Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title_short Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
title_sort genome analysis of three pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764891/
https://www.ncbi.nlm.nih.gov/pubmed/26899007
http://dx.doi.org/10.1038/ncomms10740
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