One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we establis...

Descripción completa

Detalles Bibliográficos
Autores principales: Garrels, Wiebke, Talluri, Thirumala R., Apfelbaum, Ronja, Carratalá, Yanet P., Bosch, Pablo, Pötzsch, Kerstin, Grueso, Esther, Ivics, Zoltán, Kues, Wilfried A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764937/
https://www.ncbi.nlm.nih.gov/pubmed/26905416
http://dx.doi.org/10.1038/srep21953
Descripción
Sumario:Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.

Ejemplares similares