One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we establis...

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Autores principales: Garrels, Wiebke, Talluri, Thirumala R., Apfelbaum, Ronja, Carratalá, Yanet P., Bosch, Pablo, Pötzsch, Kerstin, Grueso, Esther, Ivics, Zoltán, Kues, Wilfried A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764937/
https://www.ncbi.nlm.nih.gov/pubmed/26905416
http://dx.doi.org/10.1038/srep21953
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author Garrels, Wiebke
Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltán
Kues, Wilfried A.
author_facet Garrels, Wiebke
Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltán
Kues, Wilfried A.
author_sort Garrels, Wiebke
collection PubMed
description Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.
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spelling pubmed-47649372016-03-02 One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle Garrels, Wiebke Talluri, Thirumala R. Apfelbaum, Ronja Carratalá, Yanet P. Bosch, Pablo Pötzsch, Kerstin Grueso, Esther Ivics, Zoltán Kues, Wilfried A. Sci Rep Article Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome. Nature Publishing Group 2016-02-24 /pmc/articles/PMC4764937/ /pubmed/26905416 http://dx.doi.org/10.1038/srep21953 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Garrels, Wiebke
Talluri, Thirumala R.
Apfelbaum, Ronja
Carratalá, Yanet P.
Bosch, Pablo
Pötzsch, Kerstin
Grueso, Esther
Ivics, Zoltán
Kues, Wilfried A.
One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_full One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_fullStr One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_full_unstemmed One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_short One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle
title_sort one-step multiplex transgenesis via sleeping beauty transposition in cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764937/
https://www.ncbi.nlm.nih.gov/pubmed/26905416
http://dx.doi.org/10.1038/srep21953
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