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TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells
Multiple mechanisms exist in regulation of host responses to massive challenges from microbiota to maintain immune homeostasis in the intestines. Among these is the enriched Th17 cells in the intestines, which regulates intestinal homeostasis through induction of antimicrobial peptides and secretory...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764953/ https://www.ncbi.nlm.nih.gov/pubmed/26907705 http://dx.doi.org/10.1038/srep22040 |
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author | Liu, Han Chen, Feidi Wu, Wei Cao, Anthony T Xue, Xiaochang Yao, Suxia Evans-Marin, Heather L Li, Yan-Qing Cong, Yingzi |
author_facet | Liu, Han Chen, Feidi Wu, Wei Cao, Anthony T Xue, Xiaochang Yao, Suxia Evans-Marin, Heather L Li, Yan-Qing Cong, Yingzi |
author_sort | Liu, Han |
collection | PubMed |
description | Multiple mechanisms exist in regulation of host responses to massive challenges from microbiota to maintain immune homeostasis in the intestines. Among these is the enriched Th17 cells in the intestines, which regulates intestinal homeostasis through induction of antimicrobial peptides and secretory IgA among others. However, the means by which Th17 cells develop in response to microbiota is still not completely understood. Although both TLR5 and CD172α(+) lamina propria dendritic cells (LPDC) have been shown to promote Th17 cell development, it is still unclear whether TLR5 mediates the CD172α(+)LPDC induction of Th17 cells. By using a microbiota antigen-specific T cell reporter mouse system, we demonstrated that microbiota antigen-specific T cells developed into Th17 cells in the intestinal LP, but not in the spleen when transferred into TCRβxδ(−/−) mice. LPDCs expressed high levels of TLR5, and most CD172α(+)LPDCs also co-expressed TLR5. LPDCs produced high levels of IL-23, IL-6 and TGFβ when stimulated with commensal flagellin and promoted Th17 cell development when cultured with full-length CBir1 flagellin but not CBir1 peptide. Wild-type CD172α(+), but not CD172α(−), LPDCs induced Th17 cells, whereas TLR5-deficient LPDC did not induce Th17 cells. Our data thereby demonstrated that TLR5 mediates CD172α(+)LPDC induction of Th17 cells in the intestines. |
format | Online Article Text |
id | pubmed-4764953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47649532016-03-02 TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells Liu, Han Chen, Feidi Wu, Wei Cao, Anthony T Xue, Xiaochang Yao, Suxia Evans-Marin, Heather L Li, Yan-Qing Cong, Yingzi Sci Rep Article Multiple mechanisms exist in regulation of host responses to massive challenges from microbiota to maintain immune homeostasis in the intestines. Among these is the enriched Th17 cells in the intestines, which regulates intestinal homeostasis through induction of antimicrobial peptides and secretory IgA among others. However, the means by which Th17 cells develop in response to microbiota is still not completely understood. Although both TLR5 and CD172α(+) lamina propria dendritic cells (LPDC) have been shown to promote Th17 cell development, it is still unclear whether TLR5 mediates the CD172α(+)LPDC induction of Th17 cells. By using a microbiota antigen-specific T cell reporter mouse system, we demonstrated that microbiota antigen-specific T cells developed into Th17 cells in the intestinal LP, but not in the spleen when transferred into TCRβxδ(−/−) mice. LPDCs expressed high levels of TLR5, and most CD172α(+)LPDCs also co-expressed TLR5. LPDCs produced high levels of IL-23, IL-6 and TGFβ when stimulated with commensal flagellin and promoted Th17 cell development when cultured with full-length CBir1 flagellin but not CBir1 peptide. Wild-type CD172α(+), but not CD172α(−), LPDCs induced Th17 cells, whereas TLR5-deficient LPDC did not induce Th17 cells. Our data thereby demonstrated that TLR5 mediates CD172α(+)LPDC induction of Th17 cells in the intestines. Nature Publishing Group 2016-02-24 /pmc/articles/PMC4764953/ /pubmed/26907705 http://dx.doi.org/10.1038/srep22040 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liu, Han Chen, Feidi Wu, Wei Cao, Anthony T Xue, Xiaochang Yao, Suxia Evans-Marin, Heather L Li, Yan-Qing Cong, Yingzi TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title | TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title_full | TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title_fullStr | TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title_full_unstemmed | TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title_short | TLR5 mediates CD172α(+) intestinal lamina propria dendritic cell induction of Th17 cells |
title_sort | tlr5 mediates cd172α(+) intestinal lamina propria dendritic cell induction of th17 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4764953/ https://www.ncbi.nlm.nih.gov/pubmed/26907705 http://dx.doi.org/10.1038/srep22040 |
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