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Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France

Background and Aims The pseudometallophyte Noccaea caerulescens is an excellent model to study evolutionary processes, as it grows both on normal and on heavy-metal-rich, toxic soils. The evolution and demography of populations are critically impacted by mating system and, yet, information about the...

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Autores principales: Mousset, Mathilde, David, Patrice, Petit, Christophe, Pouzadoux, Juliette, Hatt, Clémence, Flaven, Élodie, Ronce, Ophélie, Mignot, Agnès
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765546/
https://www.ncbi.nlm.nih.gov/pubmed/26772770
http://dx.doi.org/10.1093/aob/mcv191
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author Mousset, Mathilde
David, Patrice
Petit, Christophe
Pouzadoux, Juliette
Hatt, Clémence
Flaven, Élodie
Ronce, Ophélie
Mignot, Agnès
author_facet Mousset, Mathilde
David, Patrice
Petit, Christophe
Pouzadoux, Juliette
Hatt, Clémence
Flaven, Élodie
Ronce, Ophélie
Mignot, Agnès
author_sort Mousset, Mathilde
collection PubMed
description Background and Aims The pseudometallophyte Noccaea caerulescens is an excellent model to study evolutionary processes, as it grows both on normal and on heavy-metal-rich, toxic soils. The evolution and demography of populations are critically impacted by mating system and, yet, information about the N. caerulescens mating system is limited. Methods Mean selfing rates were assessed using microsatellite loci and a robust estimation method (RMES) in five metallicolous and five non-metallicolous populations of N. caerulescens in Southern France, and this measure was replicated for two successive reproductive seasons. As a part of the study, the patterns of gene flow among populations were analysed. The mating system was then characterized at a fine spatial scale in three populations using the MLTR method on progeny arrays. Key Results The results confirm that N. caerulescens has a mixed mating system, with selfing rates ranging from 0·2 to 0·5. Selfing rates did not vary much among populations within ecotypes, but were lower in the metallicolous than in the non-metallicolous ecotype, in both seasons. Effective population size was also lower in non-metallicolous populations. Biparental inbreeding was null to moderate. Differentiation among populations was generally high, but neither ecotype nor isolation by distance explained it. Conclusions The consequences of higher selfing rates on adaptation are expected to be weak to moderate in non-metallicolous populations and they are expected to suffer less from inbreeding depression, compared to metallicolous populations.
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spelling pubmed-47655462016-03-04 Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France Mousset, Mathilde David, Patrice Petit, Christophe Pouzadoux, Juliette Hatt, Clémence Flaven, Élodie Ronce, Ophélie Mignot, Agnès Ann Bot Original Articles Background and Aims The pseudometallophyte Noccaea caerulescens is an excellent model to study evolutionary processes, as it grows both on normal and on heavy-metal-rich, toxic soils. The evolution and demography of populations are critically impacted by mating system and, yet, information about the N. caerulescens mating system is limited. Methods Mean selfing rates were assessed using microsatellite loci and a robust estimation method (RMES) in five metallicolous and five non-metallicolous populations of N. caerulescens in Southern France, and this measure was replicated for two successive reproductive seasons. As a part of the study, the patterns of gene flow among populations were analysed. The mating system was then characterized at a fine spatial scale in three populations using the MLTR method on progeny arrays. Key Results The results confirm that N. caerulescens has a mixed mating system, with selfing rates ranging from 0·2 to 0·5. Selfing rates did not vary much among populations within ecotypes, but were lower in the metallicolous than in the non-metallicolous ecotype, in both seasons. Effective population size was also lower in non-metallicolous populations. Biparental inbreeding was null to moderate. Differentiation among populations was generally high, but neither ecotype nor isolation by distance explained it. Conclusions The consequences of higher selfing rates on adaptation are expected to be weak to moderate in non-metallicolous populations and they are expected to suffer less from inbreeding depression, compared to metallicolous populations. Oxford University Press 2016-03 2016-01-15 /pmc/articles/PMC4765546/ /pubmed/26772770 http://dx.doi.org/10.1093/aob/mcv191 Text en © The Author 2016. Published by Oxford University Press on behalf of the Annals of Botany Company. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Mousset, Mathilde
David, Patrice
Petit, Christophe
Pouzadoux, Juliette
Hatt, Clémence
Flaven, Élodie
Ronce, Ophélie
Mignot, Agnès
Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title_full Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title_fullStr Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title_full_unstemmed Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title_short Lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte Noccaea caerulescens (Brassicaceae) in Southern France
title_sort lower selfing rates in metallicolous populations than in non-metallicolous populations of the pseudometallophyte noccaea caerulescens (brassicaceae) in southern france
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765546/
https://www.ncbi.nlm.nih.gov/pubmed/26772770
http://dx.doi.org/10.1093/aob/mcv191
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