Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.

To clone and examine expression profiles of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour. Thirty cDNA sequences and two genomic sequences encoding DlRan proteins were isolated from longan embryogenic cultures. Structural analysis of DlRan genes revealed that the longan Ran gene...

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Autores principales: Fang, Zhizhen, Lai, Chengchun, Zhang, Yaling, Lai, Zhongxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766155/
https://www.ncbi.nlm.nih.gov/pubmed/27026877
http://dx.doi.org/10.1186/s40064-016-1887-0
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author Fang, Zhizhen
Lai, Chengchun
Zhang, Yaling
Lai, Zhongxiong
author_facet Fang, Zhizhen
Lai, Chengchun
Zhang, Yaling
Lai, Zhongxiong
author_sort Fang, Zhizhen
collection PubMed
description To clone and examine expression profiles of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour. Thirty cDNA sequences and two genomic sequences encoding DlRan proteins were isolated from longan embryogenic cultures. Structural analysis of DlRan genes revealed that the longan Ran gene family is more expanded than that of Arabidopsis. Expression analysis of DlRan genes during somatic embryogenesis uncovered a high abundance of DlRan genes in early embryogenic cultures and heart- and torpedo-shaped embryos. The expression of DlRan genes in embryogenic calli was affected by exogenous 2,4-dichlorophenoxyacetic acid treatment. DlRan is involved in 2,4-D induced somatic embryogenesis and development of somatic embryos in longan. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-1887-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-47661552016-03-29 Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour. Fang, Zhizhen Lai, Chengchun Zhang, Yaling Lai, Zhongxiong Springerplus Research To clone and examine expression profiles of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour. Thirty cDNA sequences and two genomic sequences encoding DlRan proteins were isolated from longan embryogenic cultures. Structural analysis of DlRan genes revealed that the longan Ran gene family is more expanded than that of Arabidopsis. Expression analysis of DlRan genes during somatic embryogenesis uncovered a high abundance of DlRan genes in early embryogenic cultures and heart- and torpedo-shaped embryos. The expression of DlRan genes in embryogenic calli was affected by exogenous 2,4-dichlorophenoxyacetic acid treatment. DlRan is involved in 2,4-D induced somatic embryogenesis and development of somatic embryos in longan. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40064-016-1887-0) contains supplementary material, which is available to authorized users. Springer International Publishing 2016-02-25 /pmc/articles/PMC4766155/ /pubmed/27026877 http://dx.doi.org/10.1186/s40064-016-1887-0 Text en © Fang et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Fang, Zhizhen
Lai, Chengchun
Zhang, Yaling
Lai, Zhongxiong
Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title_full Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title_fullStr Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title_full_unstemmed Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title_short Molecular cloning, structural and expression profiling of DlRan genes during somatic embryogenesis in Dimocarpus longan Lour.
title_sort molecular cloning, structural and expression profiling of dlran genes during somatic embryogenesis in dimocarpus longan lour.
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766155/
https://www.ncbi.nlm.nih.gov/pubmed/27026877
http://dx.doi.org/10.1186/s40064-016-1887-0
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