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Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration

It is a big challenge for bone healing under osteoporotic pathological condition with impaired angiogenesis, osteogenesis and remodeling. In the present study, the effect of Ca, Mg, Si containing akermanite bioceramics (Ca(2)MgSi(2)O(7)) extract on cell proliferation, osteogenic differentiation and...

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Autores principales: Xia, Lunguo, Yin, Zhilan, Mao, Lixia, Wang, Xiuhui, Liu, Jiaqiang, Jiang, Xinquan, Zhang, Zhiyuan, Lin, Kaili, Chang, Jiang, Fang, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766478/
https://www.ncbi.nlm.nih.gov/pubmed/26911441
http://dx.doi.org/10.1038/srep22005
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author Xia, Lunguo
Yin, Zhilan
Mao, Lixia
Wang, Xiuhui
Liu, Jiaqiang
Jiang, Xinquan
Zhang, Zhiyuan
Lin, Kaili
Chang, Jiang
Fang, Bing
author_facet Xia, Lunguo
Yin, Zhilan
Mao, Lixia
Wang, Xiuhui
Liu, Jiaqiang
Jiang, Xinquan
Zhang, Zhiyuan
Lin, Kaili
Chang, Jiang
Fang, Bing
author_sort Xia, Lunguo
collection PubMed
description It is a big challenge for bone healing under osteoporotic pathological condition with impaired angiogenesis, osteogenesis and remodeling. In the present study, the effect of Ca, Mg, Si containing akermanite bioceramics (Ca(2)MgSi(2)O(7)) extract on cell proliferation, osteogenic differentiation and angiogenic factor expression of BMSCs derived from ovariectomized rats (BMSCs-OVX) as well as the expression of osteoclastogenic factors was evaluated. The results showed that akermanite could enhance cell proliferation, ALP activity, expression of Runx2, BMP-2, BSP, OPN, OCN, OPG and angiogenic factors including VEGF and ANG-1. Meanwhile, akermanite could repress expression of osteoclastogenic factors including RANKL and TNF-α. Moreover, akermanite could activate ERK, P38, AKT and STAT3 signaling pathways, while crosstalk among these signaling pathways was evident. More importantly, the effect of akermanite extract on RANKL-induced osteoclastogenesis was evaluated by TRAP staining and real-time PCR assay. The results showed that akermanite could suppress osteoclast formation and expression of TRAP, cathepsin K and NFATc1. The in vivo experiments revealed that akermanite bioceramics dramatically stimulated osteogenesis and angiogenesis in an OVX rat critical-sized calvarial defect model. All these results suggest that akermanite bioceramics with the effects of Mg and Si ions on osteogenesis, angiogenesis and osteoclastogenesis are promising biomaterials for osteoporotic bone regeneration.
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spelling pubmed-47664782016-03-02 Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration Xia, Lunguo Yin, Zhilan Mao, Lixia Wang, Xiuhui Liu, Jiaqiang Jiang, Xinquan Zhang, Zhiyuan Lin, Kaili Chang, Jiang Fang, Bing Sci Rep Article It is a big challenge for bone healing under osteoporotic pathological condition with impaired angiogenesis, osteogenesis and remodeling. In the present study, the effect of Ca, Mg, Si containing akermanite bioceramics (Ca(2)MgSi(2)O(7)) extract on cell proliferation, osteogenic differentiation and angiogenic factor expression of BMSCs derived from ovariectomized rats (BMSCs-OVX) as well as the expression of osteoclastogenic factors was evaluated. The results showed that akermanite could enhance cell proliferation, ALP activity, expression of Runx2, BMP-2, BSP, OPN, OCN, OPG and angiogenic factors including VEGF and ANG-1. Meanwhile, akermanite could repress expression of osteoclastogenic factors including RANKL and TNF-α. Moreover, akermanite could activate ERK, P38, AKT and STAT3 signaling pathways, while crosstalk among these signaling pathways was evident. More importantly, the effect of akermanite extract on RANKL-induced osteoclastogenesis was evaluated by TRAP staining and real-time PCR assay. The results showed that akermanite could suppress osteoclast formation and expression of TRAP, cathepsin K and NFATc1. The in vivo experiments revealed that akermanite bioceramics dramatically stimulated osteogenesis and angiogenesis in an OVX rat critical-sized calvarial defect model. All these results suggest that akermanite bioceramics with the effects of Mg and Si ions on osteogenesis, angiogenesis and osteoclastogenesis are promising biomaterials for osteoporotic bone regeneration. Nature Publishing Group 2016-02-25 /pmc/articles/PMC4766478/ /pubmed/26911441 http://dx.doi.org/10.1038/srep22005 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Xia, Lunguo
Yin, Zhilan
Mao, Lixia
Wang, Xiuhui
Liu, Jiaqiang
Jiang, Xinquan
Zhang, Zhiyuan
Lin, Kaili
Chang, Jiang
Fang, Bing
Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title_full Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title_fullStr Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title_full_unstemmed Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title_short Akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
title_sort akermanite bioceramics promote osteogenesis, angiogenesis and suppress osteoclastogenesis for osteoporotic bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766478/
https://www.ncbi.nlm.nih.gov/pubmed/26911441
http://dx.doi.org/10.1038/srep22005
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