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Size-selective separation of DNA fragments by using lysine-functionalized silica particles

In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica...

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Detalles Bibliográficos
Autores principales: Liu, Lingling, Guo, Zilong, Huang, Zhenzhen, Zhuang, Jiaqi, Yang, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766563/
https://www.ncbi.nlm.nih.gov/pubmed/26911527
http://dx.doi.org/10.1038/srep22029
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author Liu, Lingling
Guo, Zilong
Huang, Zhenzhen
Zhuang, Jiaqi
Yang, Wensheng
author_facet Liu, Lingling
Guo, Zilong
Huang, Zhenzhen
Zhuang, Jiaqi
Yang, Wensheng
author_sort Liu, Lingling
collection PubMed
description In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and salt concentration, therefore, the use of the lysine-functionalized silica particles allows effective separation of binary and ternary DNA mixtures, for example, two different DNA fragments with sizes of 101 and 1073 bp, 101 and 745 bp, 101 and 408 bp, respectively, and three different DNA fragments with sizes of 101, 408 and 1073 bp.
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spelling pubmed-47665632016-03-02 Size-selective separation of DNA fragments by using lysine-functionalized silica particles Liu, Lingling Guo, Zilong Huang, Zhenzhen Zhuang, Jiaqi Yang, Wensheng Sci Rep Article In this work, a facile and efficient approach has been demonstrated for size-selective separation of DNA fragments by using lysine-functionalized silica particles. At a given pH, the environmental ionic strength can be utilized to alter the electrostatic interactions of lysine-functionalized silica particles with DNA fragments and in turn the DNA fragments on the silica particle surfaces, which exhibits a clear dependence on the DNA fragment sizes. By carefully adjusting the environmental pH and salt concentration, therefore, the use of the lysine-functionalized silica particles allows effective separation of binary and ternary DNA mixtures, for example, two different DNA fragments with sizes of 101 and 1073 bp, 101 and 745 bp, 101 and 408 bp, respectively, and three different DNA fragments with sizes of 101, 408 and 1073 bp. Nature Publishing Group 2016-02-25 /pmc/articles/PMC4766563/ /pubmed/26911527 http://dx.doi.org/10.1038/srep22029 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Liu, Lingling
Guo, Zilong
Huang, Zhenzhen
Zhuang, Jiaqi
Yang, Wensheng
Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title_full Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title_fullStr Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title_full_unstemmed Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title_short Size-selective separation of DNA fragments by using lysine-functionalized silica particles
title_sort size-selective separation of dna fragments by using lysine-functionalized silica particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766563/
https://www.ncbi.nlm.nih.gov/pubmed/26911527
http://dx.doi.org/10.1038/srep22029
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