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Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis
BACKGROUND: Bovine theileriosis caused by Theileria orientalis is an emerging disease of cattle in the Asia-Pacific region where it causes a significant economic burden to meat and milk production. While host immunological responses to the lymphocyte-transforming species of Theileria, T. parva and T...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766684/ https://www.ncbi.nlm.nih.gov/pubmed/26912048 http://dx.doi.org/10.1186/s13071-016-1395-7 |
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author | Jenkins, Cheryl Bogema, Daniel R. |
author_facet | Jenkins, Cheryl Bogema, Daniel R. |
author_sort | Jenkins, Cheryl |
collection | PubMed |
description | BACKGROUND: Bovine theileriosis caused by Theileria orientalis is an emerging disease of cattle in the Asia-Pacific region where it causes a significant economic burden to meat and milk production. While host immunological responses to the lymphocyte-transforming species of Theileria, T. parva and T. annulata, have been well studied, little is known about the immune response to this non-transforming species. METHODS: We developed a recombinant antigen ELISA based on the major piroplasm surface protein (MPSP) of T. orientalis and investigated whether seroconversion to the MPSP was associated with clinical factors (anaemia), parasite burden and parasite genotype. We also examined the dynamics of seroconversion in animals acutely infected with T. orientalis. RESULTS: In cattle testing qPCR positive for T. orientalis, seroconversion was more frequent in anaemic compared to normal cattle (P < 0.0001). The ELISA ratio (ER) was highly correlated with total parasite burden as measured by qPCR (r = 0.69; P < 0.0001); however when loads of individual genotypes of the parasite were examined, only the pathogenic Ikeda genotype was highly correlated with ER. Conversely, seroconversion was less frequently detected in the presence of benign T. orientalis genotypes. Temporal measurement of the serological response, parasite burden and packed cell volume (PCV) in acutely infected animals revealed that seroconversion to the MPSP occurs within 2-3 weeks of the initial qPCR detection of the parasite and coincides with a peak in infection intensity and a declining PCV. CONCLUSION: Whether the serological response to the MPSP is immunoprotective against re-infection or recrudescence requires further investigation; however the MPSP represents a promising target for a subunit vaccine given that genetic variability within the MPSP results in differential pathogenicity of T. orientalis. |
format | Online Article Text |
id | pubmed-4766684 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47666842016-02-26 Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis Jenkins, Cheryl Bogema, Daniel R. Parasit Vectors Research BACKGROUND: Bovine theileriosis caused by Theileria orientalis is an emerging disease of cattle in the Asia-Pacific region where it causes a significant economic burden to meat and milk production. While host immunological responses to the lymphocyte-transforming species of Theileria, T. parva and T. annulata, have been well studied, little is known about the immune response to this non-transforming species. METHODS: We developed a recombinant antigen ELISA based on the major piroplasm surface protein (MPSP) of T. orientalis and investigated whether seroconversion to the MPSP was associated with clinical factors (anaemia), parasite burden and parasite genotype. We also examined the dynamics of seroconversion in animals acutely infected with T. orientalis. RESULTS: In cattle testing qPCR positive for T. orientalis, seroconversion was more frequent in anaemic compared to normal cattle (P < 0.0001). The ELISA ratio (ER) was highly correlated with total parasite burden as measured by qPCR (r = 0.69; P < 0.0001); however when loads of individual genotypes of the parasite were examined, only the pathogenic Ikeda genotype was highly correlated with ER. Conversely, seroconversion was less frequently detected in the presence of benign T. orientalis genotypes. Temporal measurement of the serological response, parasite burden and packed cell volume (PCV) in acutely infected animals revealed that seroconversion to the MPSP occurs within 2-3 weeks of the initial qPCR detection of the parasite and coincides with a peak in infection intensity and a declining PCV. CONCLUSION: Whether the serological response to the MPSP is immunoprotective against re-infection or recrudescence requires further investigation; however the MPSP represents a promising target for a subunit vaccine given that genetic variability within the MPSP results in differential pathogenicity of T. orientalis. BioMed Central 2016-02-25 /pmc/articles/PMC4766684/ /pubmed/26912048 http://dx.doi.org/10.1186/s13071-016-1395-7 Text en © Jenkins and Bogema. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Jenkins, Cheryl Bogema, Daniel R. Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title | Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title_full | Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title_fullStr | Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title_full_unstemmed | Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title_short | Factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite Theileria orientalis |
title_sort | factors associated with seroconversion to the major piroplasm surface protein of the bovine haemoparasite theileria orientalis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766684/ https://www.ncbi.nlm.nih.gov/pubmed/26912048 http://dx.doi.org/10.1186/s13071-016-1395-7 |
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