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Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice

AIM: Stress relaxant and antioxidant activities of ethanolic extract of fruit Spondias mangifera (EEFSM) and its isolated compound (Sm-01) were evaluated. The structure of Sm-01 was also elucidated. MATERIALS AND METHODS: EEFSM at two different doses of 100 and 200 mg/kg (bw)/day and Sm-01 at dose o...

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Autores principales: Arif, Muhammad, Fareed, Sheeba, Rahman, Md. Azizur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766781/
https://www.ncbi.nlm.nih.gov/pubmed/26957871
http://dx.doi.org/10.4103/0975-7406.171685
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author Arif, Muhammad
Fareed, Sheeba
Rahman, Md. Azizur
author_facet Arif, Muhammad
Fareed, Sheeba
Rahman, Md. Azizur
author_sort Arif, Muhammad
collection PubMed
description AIM: Stress relaxant and antioxidant activities of ethanolic extract of fruit Spondias mangifera (EEFSM) and its isolated compound (Sm-01) were evaluated. The structure of Sm-01 was also elucidated. MATERIALS AND METHODS: EEFSM at two different doses of 100 and 200 mg/kg (bw)/day and Sm-01 at dose of 10 mg/kg (bw)/day were screened for in vivo stress relaxant activity using anoxia stress tolerance, swimming endurance and cyclophosphamide-induced immune suppression model and in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) model. The levels of Hb, red blood cell (RBC) and white blood cell (WBC) along with organ and body weights suppressed by cyclophosphamide were estimated. The structure of Sm-01 was elucidated by spectroscopy (ultraviolet, infrared, (1)H-nuclear magnetic resonance [NMR],(13) C-NMR and mass spectrometry) and chemical analyses. RESULTS: Sm-01 was structurally elucidated as propan-1,2-dioic acid-3-carboxyl-β-D-glucopyranosyl-(6’→1”)-β-D-glucofuranoside. It was found that EEFSM and Sm-01 significantly increased the anoxia stress tolerance, swimming endurance and duration of stay on rotarod and normalized the levels of Hb, RBC, and WBC along with altered organ and body weights suppressed by cyclophosphamide. EEFSM and Sm-01 also exhibited significant antioxidant activity against DPPH free radical at the concentrations of 0.05, 0.5, and 1.0 mg/mL with obtained IC(50) of 0.32 and 0.15 mg/mL, respectively. CONCLUSIONS: These findings demonstrated that extract and Sm-01 both possess significant stress relaxant and antioxidant activities favoring its use as adaptogens. The activities of the extract may be due to the Sm-01.
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spelling pubmed-47667812016-03-08 Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice Arif, Muhammad Fareed, Sheeba Rahman, Md. Azizur J Pharm Bioallied Sci Original Article AIM: Stress relaxant and antioxidant activities of ethanolic extract of fruit Spondias mangifera (EEFSM) and its isolated compound (Sm-01) were evaluated. The structure of Sm-01 was also elucidated. MATERIALS AND METHODS: EEFSM at two different doses of 100 and 200 mg/kg (bw)/day and Sm-01 at dose of 10 mg/kg (bw)/day were screened for in vivo stress relaxant activity using anoxia stress tolerance, swimming endurance and cyclophosphamide-induced immune suppression model and in vitro antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) model. The levels of Hb, red blood cell (RBC) and white blood cell (WBC) along with organ and body weights suppressed by cyclophosphamide were estimated. The structure of Sm-01 was elucidated by spectroscopy (ultraviolet, infrared, (1)H-nuclear magnetic resonance [NMR],(13) C-NMR and mass spectrometry) and chemical analyses. RESULTS: Sm-01 was structurally elucidated as propan-1,2-dioic acid-3-carboxyl-β-D-glucopyranosyl-(6’→1”)-β-D-glucofuranoside. It was found that EEFSM and Sm-01 significantly increased the anoxia stress tolerance, swimming endurance and duration of stay on rotarod and normalized the levels of Hb, RBC, and WBC along with altered organ and body weights suppressed by cyclophosphamide. EEFSM and Sm-01 also exhibited significant antioxidant activity against DPPH free radical at the concentrations of 0.05, 0.5, and 1.0 mg/mL with obtained IC(50) of 0.32 and 0.15 mg/mL, respectively. CONCLUSIONS: These findings demonstrated that extract and Sm-01 both possess significant stress relaxant and antioxidant activities favoring its use as adaptogens. The activities of the extract may be due to the Sm-01. Medknow Publications & Media Pvt Ltd 2016 /pmc/articles/PMC4766781/ /pubmed/26957871 http://dx.doi.org/10.4103/0975-7406.171685 Text en Copyright: © Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Arif, Muhammad
Fareed, Sheeba
Rahman, Md. Azizur
Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title_full Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title_fullStr Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title_full_unstemmed Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title_short Stress relaxant and antioxidant activities of acid glycoside from Spondias mangifera fruit against physically and chemically challenged albino mice
title_sort stress relaxant and antioxidant activities of acid glycoside from spondias mangifera fruit against physically and chemically challenged albino mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766781/
https://www.ncbi.nlm.nih.gov/pubmed/26957871
http://dx.doi.org/10.4103/0975-7406.171685
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