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Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran
Background: Pseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. Material and methods: 160 isolates of P....
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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German Medical Science GMS Publishing House
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766921/ https://www.ncbi.nlm.nih.gov/pubmed/26958458 http://dx.doi.org/10.3205/dgkh000264 |
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author | Azimi, Somayeh Kafil, Hossein Samadi Baghi, Hossein Bannazadeh Shokrian, Saeed Najaf, Khadijeh Asgharzadeh, Mohammad Yousefi, Mehdi Shahrivar, Firooz Aghazadeh, Mohammad |
author_facet | Azimi, Somayeh Kafil, Hossein Samadi Baghi, Hossein Bannazadeh Shokrian, Saeed Najaf, Khadijeh Asgharzadeh, Mohammad Yousefi, Mehdi Shahrivar, Firooz Aghazadeh, Mohammad |
author_sort | Azimi, Somayeh |
collection | PubMed |
description | Background: Pseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. Material and methods: 160 isolates of P. aeruginosa were collected and identified by biochemical tests and were characterized for antibiotic resistance. Biofilm production was evaluated by microtiter plate assay and the presence of exo genes was evaluated by allele-specific PCR (polymerase chain reaction). Chi-square test was used for statistical analysis. Results: The most effective antibiotics against isolates were colistin and polymyxin B. 87% of the isolates were biofilm producers of which 69% were strongly biofilm producers. 55% of the isolates carried exoY, 52% of the isolates carried exoU, and 26.3% and 5% carried exoS and exoT, respectively. Conclusion: Our findings showed different distribution of exo genes in clinical isolates of P. aeruginosa in Northwest Iran. ExoS and exoU were more prevalent in non-biofilm producers and exoY was more prevalent in biofilm producer isolates. These results might indicate the importance of exoY in biofilm production of Pseudomonas aeruginosa. |
format | Online Article Text |
id | pubmed-4766921 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | German Medical Science GMS Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-47669212016-03-08 Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran Azimi, Somayeh Kafil, Hossein Samadi Baghi, Hossein Bannazadeh Shokrian, Saeed Najaf, Khadijeh Asgharzadeh, Mohammad Yousefi, Mehdi Shahrivar, Firooz Aghazadeh, Mohammad GMS Hyg Infect Control Article Background: Pseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. Material and methods: 160 isolates of P. aeruginosa were collected and identified by biochemical tests and were characterized for antibiotic resistance. Biofilm production was evaluated by microtiter plate assay and the presence of exo genes was evaluated by allele-specific PCR (polymerase chain reaction). Chi-square test was used for statistical analysis. Results: The most effective antibiotics against isolates were colistin and polymyxin B. 87% of the isolates were biofilm producers of which 69% were strongly biofilm producers. 55% of the isolates carried exoY, 52% of the isolates carried exoU, and 26.3% and 5% carried exoS and exoT, respectively. Conclusion: Our findings showed different distribution of exo genes in clinical isolates of P. aeruginosa in Northwest Iran. ExoS and exoU were more prevalent in non-biofilm producers and exoY was more prevalent in biofilm producer isolates. These results might indicate the importance of exoY in biofilm production of Pseudomonas aeruginosa. German Medical Science GMS Publishing House 2016-02-22 /pmc/articles/PMC4766921/ /pubmed/26958458 http://dx.doi.org/10.3205/dgkh000264 Text en Copyright © 2016 Azimi et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License. |
spellingShingle | Article Azimi, Somayeh Kafil, Hossein Samadi Baghi, Hossein Bannazadeh Shokrian, Saeed Najaf, Khadijeh Asgharzadeh, Mohammad Yousefi, Mehdi Shahrivar, Firooz Aghazadeh, Mohammad Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title | Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title_full | Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title_fullStr | Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title_full_unstemmed | Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title_short | Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran |
title_sort | presence of exoy, exos, exou and exot genes, antibiotic resistance and biofilm production among pseudomonas aeruginosa isolates in northwest iran |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766921/ https://www.ncbi.nlm.nih.gov/pubmed/26958458 http://dx.doi.org/10.3205/dgkh000264 |
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