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New DAG and cAMP Sensors Optimized for Live-Cell Assays in Automated Laboratories

Protein-based, fluorescent biosensors power basic research on cell signaling in health and disease, but their use in automated laboratories is limited. We have now created two live-cell assays, one for diacyl glycerol and another for cAMP, that are robust (Z′ > 0.7) and easily deployed on standar...

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Detalles Bibliográficos
Autores principales: Tewson, Paul H., Martinka, Scott, Shaner, Nathan C., Hughes, Thomas E., Quinn, Anne Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4766961/
https://www.ncbi.nlm.nih.gov/pubmed/26657040
http://dx.doi.org/10.1177/1087057115618608