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High frame-rate resolution of cell division during Candida albicans filamentation

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed th...

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Detalles Bibliográficos
Autores principales: Thomson, Darren D., Berman, Judith, Brand, Alexandra C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767323/
https://www.ncbi.nlm.nih.gov/pubmed/26854071
http://dx.doi.org/10.1016/j.fgb.2016.02.001
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author Thomson, Darren D.
Berman, Judith
Brand, Alexandra C.
author_facet Thomson, Darren D.
Berman, Judith
Brand, Alexandra C.
author_sort Thomson, Darren D.
collection PubMed
description The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.
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spelling pubmed-47673232016-03-01 High frame-rate resolution of cell division during Candida albicans filamentation Thomson, Darren D. Berman, Judith Brand, Alexandra C. Fungal Genet Biol Video Article The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1 frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division. Academic Press 2016-03 /pmc/articles/PMC4767323/ /pubmed/26854071 http://dx.doi.org/10.1016/j.fgb.2016.02.001 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Video Article
Thomson, Darren D.
Berman, Judith
Brand, Alexandra C.
High frame-rate resolution of cell division during Candida albicans filamentation
title High frame-rate resolution of cell division during Candida albicans filamentation
title_full High frame-rate resolution of cell division during Candida albicans filamentation
title_fullStr High frame-rate resolution of cell division during Candida albicans filamentation
title_full_unstemmed High frame-rate resolution of cell division during Candida albicans filamentation
title_short High frame-rate resolution of cell division during Candida albicans filamentation
title_sort high frame-rate resolution of cell division during candida albicans filamentation
topic Video Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767323/
https://www.ncbi.nlm.nih.gov/pubmed/26854071
http://dx.doi.org/10.1016/j.fgb.2016.02.001
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