An informatic framework for decoding protein complexes by top-down mass spectrometry

Efforts to map the human protein interactome have resulted in information about hundreds to thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we combined the CORUM and Uni...

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Autores principales: Skinner, Owen S., Havugimana, Pierre C., Haverland, Nicole A., Fornelli, Luca, Early, Bryan P., Greer, Joseph B., Fellers, Ryan T., Durbin, Kenneth R., Do Vale, Luis H. F., Melani, Rafael D., Seckler, Henrique S., Nelp, Micah T., Belov, Mikhail E., Horning, Stevan R., Makarov, Alexander A., LeDuc, Richard D., Bandarian, Vahe, Compton, Philip D., Kelleher, Neil L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767540/
https://www.ncbi.nlm.nih.gov/pubmed/26780093
http://dx.doi.org/10.1038/nmeth.3731
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author Skinner, Owen S.
Havugimana, Pierre C.
Haverland, Nicole A.
Fornelli, Luca
Early, Bryan P.
Greer, Joseph B.
Fellers, Ryan T.
Durbin, Kenneth R.
Do Vale, Luis H. F.
Melani, Rafael D.
Seckler, Henrique S.
Nelp, Micah T.
Belov, Mikhail E.
Horning, Stevan R.
Makarov, Alexander A.
LeDuc, Richard D.
Bandarian, Vahe
Compton, Philip D.
Kelleher, Neil L.
author_facet Skinner, Owen S.
Havugimana, Pierre C.
Haverland, Nicole A.
Fornelli, Luca
Early, Bryan P.
Greer, Joseph B.
Fellers, Ryan T.
Durbin, Kenneth R.
Do Vale, Luis H. F.
Melani, Rafael D.
Seckler, Henrique S.
Nelp, Micah T.
Belov, Mikhail E.
Horning, Stevan R.
Makarov, Alexander A.
LeDuc, Richard D.
Bandarian, Vahe
Compton, Philip D.
Kelleher, Neil L.
author_sort Skinner, Owen S.
collection PubMed
description Efforts to map the human protein interactome have resulted in information about hundreds to thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we combined the CORUM and UniProt databases to create candidates for an error-tolerant search engine designed for hierarchical top-down analyses, identification, and scoring of multi-proteoform complexes by native mass spectrometry.
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spelling pubmed-47675402016-07-18 An informatic framework for decoding protein complexes by top-down mass spectrometry Skinner, Owen S. Havugimana, Pierre C. Haverland, Nicole A. Fornelli, Luca Early, Bryan P. Greer, Joseph B. Fellers, Ryan T. Durbin, Kenneth R. Do Vale, Luis H. F. Melani, Rafael D. Seckler, Henrique S. Nelp, Micah T. Belov, Mikhail E. Horning, Stevan R. Makarov, Alexander A. LeDuc, Richard D. Bandarian, Vahe Compton, Philip D. Kelleher, Neil L. Nat Methods Article Efforts to map the human protein interactome have resulted in information about hundreds to thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we combined the CORUM and UniProt databases to create candidates for an error-tolerant search engine designed for hierarchical top-down analyses, identification, and scoring of multi-proteoform complexes by native mass spectrometry. 2016-01-18 2016-03 /pmc/articles/PMC4767540/ /pubmed/26780093 http://dx.doi.org/10.1038/nmeth.3731 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Skinner, Owen S.
Havugimana, Pierre C.
Haverland, Nicole A.
Fornelli, Luca
Early, Bryan P.
Greer, Joseph B.
Fellers, Ryan T.
Durbin, Kenneth R.
Do Vale, Luis H. F.
Melani, Rafael D.
Seckler, Henrique S.
Nelp, Micah T.
Belov, Mikhail E.
Horning, Stevan R.
Makarov, Alexander A.
LeDuc, Richard D.
Bandarian, Vahe
Compton, Philip D.
Kelleher, Neil L.
An informatic framework for decoding protein complexes by top-down mass spectrometry
title An informatic framework for decoding protein complexes by top-down mass spectrometry
title_full An informatic framework for decoding protein complexes by top-down mass spectrometry
title_fullStr An informatic framework for decoding protein complexes by top-down mass spectrometry
title_full_unstemmed An informatic framework for decoding protein complexes by top-down mass spectrometry
title_short An informatic framework for decoding protein complexes by top-down mass spectrometry
title_sort informatic framework for decoding protein complexes by top-down mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767540/
https://www.ncbi.nlm.nih.gov/pubmed/26780093
http://dx.doi.org/10.1038/nmeth.3731
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