Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()

OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at −80 °C. Mesenchymal stromal cells from the bon...

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Autores principales: Augusto, Lívia Maria Mendonça, Aguiar, Diego Pinheiro, Bonfim, Danielle Cabral, dos Santos Cavalcanti, Amanda, Casado, Priscila Ladeira, Duarte, Maria Eugênia Leite
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767843/
https://www.ncbi.nlm.nih.gov/pubmed/26962503
http://dx.doi.org/10.1016/j.rboe.2015.12.013
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author Augusto, Lívia Maria Mendonça
Aguiar, Diego Pinheiro
Bonfim, Danielle Cabral
dos Santos Cavalcanti, Amanda
Casado, Priscila Ladeira
Duarte, Maria Eugênia Leite
author_facet Augusto, Lívia Maria Mendonça
Aguiar, Diego Pinheiro
Bonfim, Danielle Cabral
dos Santos Cavalcanti, Amanda
Casado, Priscila Ladeira
Duarte, Maria Eugênia Leite
author_sort Augusto, Lívia Maria Mendonça
collection PubMed
description OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at −80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.
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spelling pubmed-47678432016-03-09 Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes() Augusto, Lívia Maria Mendonça Aguiar, Diego Pinheiro Bonfim, Danielle Cabral dos Santos Cavalcanti, Amanda Casado, Priscila Ladeira Duarte, Maria Eugênia Leite Rev Bras Ortop Original Article OBJECTIVE: This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract. METHODS: Bovine tendons were used for preparation of the extract and were stored at −80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR. RESULTS: The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression. CONCLUSION: Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes. Elsevier 2016-01-06 /pmc/articles/PMC4767843/ /pubmed/26962503 http://dx.doi.org/10.1016/j.rboe.2015.12.013 Text en © 2015 Sociedade Brasileira de Ortopedia e Traumatologia. Published by Elsevier Editora Ltda. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Augusto, Lívia Maria Mendonça
Aguiar, Diego Pinheiro
Bonfim, Danielle Cabral
dos Santos Cavalcanti, Amanda
Casado, Priscila Ladeira
Duarte, Maria Eugênia Leite
Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title_full Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title_fullStr Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title_full_unstemmed Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title_short Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
title_sort mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes()
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767843/
https://www.ncbi.nlm.nih.gov/pubmed/26962503
http://dx.doi.org/10.1016/j.rboe.2015.12.013
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