Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid te...

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Autores principales: Scriven, James E., Graham, Lisa M., Schutz, Charlotte, Scriba, Thomas J., Wilkinson, Robert J., Boulware, David R., Meintjes, Graeme, Lalloo, David G., Urban, Britta C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Mycology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767993/
https://www.ncbi.nlm.nih.gov/pubmed/26719441
http://dx.doi.org/10.1128/JCM.03002-15
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author Scriven, James E.
Graham, Lisa M.
Schutz, Charlotte
Scriba, Thomas J.
Wilkinson, Robert J.
Boulware, David R.
Meintjes, Graeme
Lalloo, David G.
Urban, Britta C.
author_facet Scriven, James E.
Graham, Lisa M.
Schutz, Charlotte
Scriba, Thomas J.
Wilkinson, Robert J.
Boulware, David R.
Meintjes, Graeme
Lalloo, David G.
Urban, Britta C.
author_sort Scriven, James E.
collection PubMed
description Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.
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spelling pubmed-47679932016-03-02 Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis Scriven, James E. Graham, Lisa M. Schutz, Charlotte Scriba, Thomas J. Wilkinson, Robert J. Boulware, David R. Meintjes, Graeme Lalloo, David G. Urban, Britta C. J Clin Microbiol Mycology Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue. American Society for Microbiology 2016-02-25 2016-03 /pmc/articles/PMC4767993/ /pubmed/26719441 http://dx.doi.org/10.1128/JCM.03002-15 Text en Copyright © 2016 Scriven et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Mycology
Scriven, James E.
Graham, Lisa M.
Schutz, Charlotte
Scriba, Thomas J.
Wilkinson, Robert J.
Boulware, David R.
Meintjes, Graeme
Lalloo, David G.
Urban, Britta C.
Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title_full Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title_fullStr Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title_full_unstemmed Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title_short Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis
title_sort flow cytometry to assess cerebrospinal fluid fungal burden in cryptococcal meningitis
topic Mycology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767993/
https://www.ncbi.nlm.nih.gov/pubmed/26719441
http://dx.doi.org/10.1128/JCM.03002-15
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