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Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals
The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackieviru...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768086/ https://www.ncbi.nlm.nih.gov/pubmed/26917423 http://dx.doi.org/10.1038/srep21979 |
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author | Gao, Caixia Ding, Yingying Zhou, Peng Feng, Jiaojiao Qian, Baohua Lin, Ziyu Wang, Lili Wang, Jinhong Zhao, Chunyan Li, Xiangyu Cao, Mingmei Peng, Heng Rui, Bing Pan, Wei |
author_facet | Gao, Caixia Ding, Yingying Zhou, Peng Feng, Jiaojiao Qian, Baohua Lin, Ziyu Wang, Lili Wang, Jinhong Zhao, Chunyan Li, Xiangyu Cao, Mingmei Peng, Heng Rui, Bing Pan, Wei |
author_sort | Gao, Caixia |
collection | PubMed |
description | The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA. |
format | Online Article Text |
id | pubmed-4768086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47680862016-03-02 Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals Gao, Caixia Ding, Yingying Zhou, Peng Feng, Jiaojiao Qian, Baohua Lin, Ziyu Wang, Lili Wang, Jinhong Zhao, Chunyan Li, Xiangyu Cao, Mingmei Peng, Heng Rui, Bing Pan, Wei Sci Rep Article The overall serological prevalence of EV infections based on ELISA remains unknown. In the present study, the antibody responses against VP1 of the EV-A species (enterovirus 71 (EV71), Coxsackievirus A16 (CA16), Coxsackievirus A5 (CA5) and Coxsackievirus A6 (CA6)), of the EV-B species (Coxsackievirus B3 (CB3)), and of the EV-C species (Poliovirus 1 (PV1)) were detected and analyzed by a NEIBM (novel evolved immunoglobulin-binding molecule)-based ELISA in Shanghai blood donors. The serological prevalence of anti-CB3 VP1 antibodies was demonstrated to show the highest level, with anti-PV1 VP1 antibodies at the second highest level, and anti-CA5, CA6, CA16 and EV71 VP1 antibodies at a comparatively low level. All reactions were significantly correlated at different levels, which were approximately proportional to their sequence similarities. Antibody responses against EV71 VP1 showed obvious differences with responses against other EV-A viruses. Obvious differences in antibody responses between August 2013 and May 2014 were revealed. These findings are the first to describe the detailed information of the serological prevalence of human antibody responses against the VP1 of EV-A, B and C viruses, and could be helpful for understanding of the ubiquity of EV infections and for identifying an effective approach for seroepidemiological surveillance based on ELISA. Nature Publishing Group 2016-02-26 /pmc/articles/PMC4768086/ /pubmed/26917423 http://dx.doi.org/10.1038/srep21979 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Gao, Caixia Ding, Yingying Zhou, Peng Feng, Jiaojiao Qian, Baohua Lin, Ziyu Wang, Lili Wang, Jinhong Zhao, Chunyan Li, Xiangyu Cao, Mingmei Peng, Heng Rui, Bing Pan, Wei Serological detection and analysis of anti-VP1 responses against various enteroviruses (EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title | Serological detection and analysis of anti-VP1 responses against various enteroviruses
(EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title_full | Serological detection and analysis of anti-VP1 responses against various enteroviruses
(EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title_fullStr | Serological detection and analysis of anti-VP1 responses against various enteroviruses
(EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title_full_unstemmed | Serological detection and analysis of anti-VP1 responses against various enteroviruses
(EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title_short | Serological detection and analysis of anti-VP1 responses against various enteroviruses
(EV) (EV-A, EV-B and EV-C) in Chinese individuals |
title_sort | serological detection and analysis of anti-vp1 responses against various enteroviruses
(ev) (ev-a, ev-b and ev-c) in chinese individuals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768086/ https://www.ncbi.nlm.nih.gov/pubmed/26917423 http://dx.doi.org/10.1038/srep21979 |
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