Cargando…

Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil

Stingrays commonly cause human envenoming related accidents in populations of the sea, near rivers and lakes. Transcriptomic profiles have been used to elucidate components of animal venom, since they are capable of providing molecular information on the biology of the animal and could have biomedic...

Descripción completa

Detalles Bibliográficos
Autores principales: Júnior, Nelson Gomes de Oliveira, Fernandes, Gabriel da Rocha, Cardoso, Marlon Henrique, Costa, Fabrício F., Cândido, Elizabete de Souza, Neto, Domingos Garrone, Mortari, Márcia Renata, Schwartz, Elisabeth Ferroni, Franco, Octávio Luiz, de Alencar, Sérgio Amorim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768133/
https://www.ncbi.nlm.nih.gov/pubmed/26916342
http://dx.doi.org/10.1038/srep21935
_version_ 1782417896811528192
author Júnior, Nelson Gomes de Oliveira
Fernandes, Gabriel da Rocha
Cardoso, Marlon Henrique
Costa, Fabrício F.
Cândido, Elizabete de Souza
Neto, Domingos Garrone
Mortari, Márcia Renata
Schwartz, Elisabeth Ferroni
Franco, Octávio Luiz
de Alencar, Sérgio Amorim
author_facet Júnior, Nelson Gomes de Oliveira
Fernandes, Gabriel da Rocha
Cardoso, Marlon Henrique
Costa, Fabrício F.
Cândido, Elizabete de Souza
Neto, Domingos Garrone
Mortari, Márcia Renata
Schwartz, Elisabeth Ferroni
Franco, Octávio Luiz
de Alencar, Sérgio Amorim
author_sort Júnior, Nelson Gomes de Oliveira
collection PubMed
description Stingrays commonly cause human envenoming related accidents in populations of the sea, near rivers and lakes. Transcriptomic profiles have been used to elucidate components of animal venom, since they are capable of providing molecular information on the biology of the animal and could have biomedical applications. In this study, we elucidated the transcriptomic profile of the venom glands from two different freshwater stingray species that are endemic to the Paraná-Paraguay basin in Brazil, Potamotrygon amandae and Potamotrygon falkneri. Using RNA-Seq, we identified species-specific transcripts and overlapping proteins in the venom gland of both species. Among the transcripts related with envenoming, high abundance of hyaluronidases was observed in both species. In addition, we built three-dimensional homology models based on several venom transcripts identified. Our study represents a significant improvement in the information about the venoms employed by these two species and their molecular characteristics. Moreover, the information generated by our group helps in a better understanding of the biology of freshwater cartilaginous fishes and offers clues for the development of clinical treatments for stingray envenoming in Brazil and around the world. Finally, our results might have biomedical implications in developing treatments for complex diseases.
format Online
Article
Text
id pubmed-4768133
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-47681332016-03-02 Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil Júnior, Nelson Gomes de Oliveira Fernandes, Gabriel da Rocha Cardoso, Marlon Henrique Costa, Fabrício F. Cândido, Elizabete de Souza Neto, Domingos Garrone Mortari, Márcia Renata Schwartz, Elisabeth Ferroni Franco, Octávio Luiz de Alencar, Sérgio Amorim Sci Rep Article Stingrays commonly cause human envenoming related accidents in populations of the sea, near rivers and lakes. Transcriptomic profiles have been used to elucidate components of animal venom, since they are capable of providing molecular information on the biology of the animal and could have biomedical applications. In this study, we elucidated the transcriptomic profile of the venom glands from two different freshwater stingray species that are endemic to the Paraná-Paraguay basin in Brazil, Potamotrygon amandae and Potamotrygon falkneri. Using RNA-Seq, we identified species-specific transcripts and overlapping proteins in the venom gland of both species. Among the transcripts related with envenoming, high abundance of hyaluronidases was observed in both species. In addition, we built three-dimensional homology models based on several venom transcripts identified. Our study represents a significant improvement in the information about the venoms employed by these two species and their molecular characteristics. Moreover, the information generated by our group helps in a better understanding of the biology of freshwater cartilaginous fishes and offers clues for the development of clinical treatments for stingray envenoming in Brazil and around the world. Finally, our results might have biomedical implications in developing treatments for complex diseases. Nature Publishing Group 2016-02-26 /pmc/articles/PMC4768133/ /pubmed/26916342 http://dx.doi.org/10.1038/srep21935 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Júnior, Nelson Gomes de Oliveira
Fernandes, Gabriel da Rocha
Cardoso, Marlon Henrique
Costa, Fabrício F.
Cândido, Elizabete de Souza
Neto, Domingos Garrone
Mortari, Márcia Renata
Schwartz, Elisabeth Ferroni
Franco, Octávio Luiz
de Alencar, Sérgio Amorim
Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title_full Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title_fullStr Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title_full_unstemmed Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title_short Venom gland transcriptome analyses of two freshwater stingrays (Myliobatiformes: Potamotrygonidae) from Brazil
title_sort venom gland transcriptome analyses of two freshwater stingrays (myliobatiformes: potamotrygonidae) from brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768133/
https://www.ncbi.nlm.nih.gov/pubmed/26916342
http://dx.doi.org/10.1038/srep21935
work_keys_str_mv AT juniornelsongomesdeoliveira venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT fernandesgabrieldarocha venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT cardosomarlonhenrique venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT costafabriciof venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT candidoelizabetedesouza venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT netodomingosgarrone venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT mortarimarciarenata venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT schwartzelisabethferroni venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT francooctavioluiz venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil
AT dealencarsergioamorim venomglandtranscriptomeanalysesoftwofreshwaterstingraysmyliobatiformespotamotrygonidaefrombrazil