Cargando…
[(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing prec...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768225/ https://www.ncbi.nlm.nih.gov/pubmed/25708744 http://dx.doi.org/10.1007/s11307-015-0831-y |
_version_ | 1782417912174215168 |
---|---|
author | Peeters, Sarah G. J. A. Dubois, Ludwig Lieuwes, Natasja G. Laan, Dennis Mooijer, Martien Schuit, Robert C. Vullo, Daniela Supuran, Claudiu T. Eriksson, Jonas Windhorst, Albert D. Lambin, Philippe |
author_facet | Peeters, Sarah G. J. A. Dubois, Ludwig Lieuwes, Natasja G. Laan, Dennis Mooijer, Martien Schuit, Robert C. Vullo, Daniela Supuran, Claudiu T. Eriksson, Jonas Windhorst, Albert D. Lambin, Philippe |
author_sort | Peeters, Sarah G. J. A. |
collection | PubMed |
description | PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing preclinical human tumor models. PROCEDURES: U373 and HT29 tumor-bearing animals were injected with [(18)F]VM4-037 and underwent microPET imaging up to 4 h post-injection (p.i.). Biodistribution throughout the different organs was assessed at 2 and 4 h p.i. using gamma counting. RESULTS: MicroPET imaging showed high [(18)F]VM4-037 uptake in the abdominal region, and biodistribution revealed high radioactivity in the kidney, ileum, colon, liver, stomach, and bladder. Although high CAIX expression was confirmed in both tumor models, tumor uptake assessed with microPET and biodistribution experiments was comparable to background tissues. CONCLUSIONS: In this study, [(18)F]VM4-037 does not specifically accumulate in CAIX-expressing tumors, indicating that the tracer is not suitable for the detection of CAIX. |
format | Online Article Text |
id | pubmed-4768225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-47682252016-03-29 [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models Peeters, Sarah G. J. A. Dubois, Ludwig Lieuwes, Natasja G. Laan, Dennis Mooijer, Martien Schuit, Robert C. Vullo, Daniela Supuran, Claudiu T. Eriksson, Jonas Windhorst, Albert D. Lambin, Philippe Mol Imaging Biol Brief Article PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing preclinical human tumor models. PROCEDURES: U373 and HT29 tumor-bearing animals were injected with [(18)F]VM4-037 and underwent microPET imaging up to 4 h post-injection (p.i.). Biodistribution throughout the different organs was assessed at 2 and 4 h p.i. using gamma counting. RESULTS: MicroPET imaging showed high [(18)F]VM4-037 uptake in the abdominal region, and biodistribution revealed high radioactivity in the kidney, ileum, colon, liver, stomach, and bladder. Although high CAIX expression was confirmed in both tumor models, tumor uptake assessed with microPET and biodistribution experiments was comparable to background tissues. CONCLUSIONS: In this study, [(18)F]VM4-037 does not specifically accumulate in CAIX-expressing tumors, indicating that the tracer is not suitable for the detection of CAIX. Springer US 2015-02-24 2015 /pmc/articles/PMC4768225/ /pubmed/25708744 http://dx.doi.org/10.1007/s11307-015-0831-y Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Brief Article Peeters, Sarah G. J. A. Dubois, Ludwig Lieuwes, Natasja G. Laan, Dennis Mooijer, Martien Schuit, Robert C. Vullo, Daniela Supuran, Claudiu T. Eriksson, Jonas Windhorst, Albert D. Lambin, Philippe [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title | [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title_full | [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title_fullStr | [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title_full_unstemmed | [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title_short | [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models |
title_sort | [(18)f]vm4-037 micropet imaging and biodistribution of two in vivo caix-expressing tumor models |
topic | Brief Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768225/ https://www.ncbi.nlm.nih.gov/pubmed/25708744 http://dx.doi.org/10.1007/s11307-015-0831-y |
work_keys_str_mv | AT peeterssarahgja 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT duboisludwig 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT lieuwesnatasjag 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT laandennis 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT mooijermartien 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT schuitrobertc 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT vullodaniela 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT supuranclaudiut 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT erikssonjonas 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT windhorstalbertd 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels AT lambinphilippe 18fvm4037micropetimagingandbiodistributionoftwoinvivocaixexpressingtumormodels |