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[(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models

PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing prec...

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Autores principales: Peeters, Sarah G. J. A., Dubois, Ludwig, Lieuwes, Natasja G., Laan, Dennis, Mooijer, Martien, Schuit, Robert C., Vullo, Daniela, Supuran, Claudiu T., Eriksson, Jonas, Windhorst, Albert D., Lambin, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768225/
https://www.ncbi.nlm.nih.gov/pubmed/25708744
http://dx.doi.org/10.1007/s11307-015-0831-y
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author Peeters, Sarah G. J. A.
Dubois, Ludwig
Lieuwes, Natasja G.
Laan, Dennis
Mooijer, Martien
Schuit, Robert C.
Vullo, Daniela
Supuran, Claudiu T.
Eriksson, Jonas
Windhorst, Albert D.
Lambin, Philippe
author_facet Peeters, Sarah G. J. A.
Dubois, Ludwig
Lieuwes, Natasja G.
Laan, Dennis
Mooijer, Martien
Schuit, Robert C.
Vullo, Daniela
Supuran, Claudiu T.
Eriksson, Jonas
Windhorst, Albert D.
Lambin, Philippe
author_sort Peeters, Sarah G. J. A.
collection PubMed
description PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing preclinical human tumor models. PROCEDURES: U373 and HT29 tumor-bearing animals were injected with [(18)F]VM4-037 and underwent microPET imaging up to 4 h post-injection (p.i.). Biodistribution throughout the different organs was assessed at 2 and 4 h p.i. using gamma counting. RESULTS: MicroPET imaging showed high [(18)F]VM4-037 uptake in the abdominal region, and biodistribution revealed high radioactivity in the kidney, ileum, colon, liver, stomach, and bladder. Although high CAIX expression was confirmed in both tumor models, tumor uptake assessed with microPET and biodistribution experiments was comparable to background tissues. CONCLUSIONS: In this study, [(18)F]VM4-037 does not specifically accumulate in CAIX-expressing tumors, indicating that the tracer is not suitable for the detection of CAIX.
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spelling pubmed-47682252016-03-29 [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models Peeters, Sarah G. J. A. Dubois, Ludwig Lieuwes, Natasja G. Laan, Dennis Mooijer, Martien Schuit, Robert C. Vullo, Daniela Supuran, Claudiu T. Eriksson, Jonas Windhorst, Albert D. Lambin, Philippe Mol Imaging Biol Brief Article PURPOSE: [(18)F]VM4-037 was recently developed as a positron emission tomography (PET) tracer for the detection of carbonic anhydrase IX (CAIX), a tumor-specific protein upregulated under hypoxic conditions. In this study, the accumulation of [(18)F]VM4-037 was determined in two CAIX-expressing preclinical human tumor models. PROCEDURES: U373 and HT29 tumor-bearing animals were injected with [(18)F]VM4-037 and underwent microPET imaging up to 4 h post-injection (p.i.). Biodistribution throughout the different organs was assessed at 2 and 4 h p.i. using gamma counting. RESULTS: MicroPET imaging showed high [(18)F]VM4-037 uptake in the abdominal region, and biodistribution revealed high radioactivity in the kidney, ileum, colon, liver, stomach, and bladder. Although high CAIX expression was confirmed in both tumor models, tumor uptake assessed with microPET and biodistribution experiments was comparable to background tissues. CONCLUSIONS: In this study, [(18)F]VM4-037 does not specifically accumulate in CAIX-expressing tumors, indicating that the tracer is not suitable for the detection of CAIX. Springer US 2015-02-24 2015 /pmc/articles/PMC4768225/ /pubmed/25708744 http://dx.doi.org/10.1007/s11307-015-0831-y Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Brief Article
Peeters, Sarah G. J. A.
Dubois, Ludwig
Lieuwes, Natasja G.
Laan, Dennis
Mooijer, Martien
Schuit, Robert C.
Vullo, Daniela
Supuran, Claudiu T.
Eriksson, Jonas
Windhorst, Albert D.
Lambin, Philippe
[(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title_full [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title_fullStr [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title_full_unstemmed [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title_short [(18)F]VM4-037 MicroPET Imaging and Biodistribution of Two In Vivo CAIX-Expressing Tumor Models
title_sort [(18)f]vm4-037 micropet imaging and biodistribution of two in vivo caix-expressing tumor models
topic Brief Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768225/
https://www.ncbi.nlm.nih.gov/pubmed/25708744
http://dx.doi.org/10.1007/s11307-015-0831-y
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