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The importance of selecting the appropriate reference genes for quantitative real time PCR as illustrated using colon cancer cells and tissue

Quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) remains the most sensitive technique for nucleic acid quantification. Its popularity is reflected in the remarkable number of publications reporting RT-qPCR data. Careful normalisation within RT-qPCR studies is imperati...

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Detalles Bibliográficos
Autores principales:	Dowling, Catríona M., Walsh, Dara, Coffey, John C., Kiely, Patrick A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	F1000Research 2016
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768652/ https://www.ncbi.nlm.nih.gov/pubmed/26962435 http://dx.doi.org/10.12688/f1000research.7656.2

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