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Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging
Redd1, also known as RTP801/Dig2/DDIT4, is a stress-induced protein and a negative regulator of mammalian target of rapamycin (mTOR). Redd1 is also closely associated with oxidative stress and DNA damage. In the present study, age-related changes in the protein expression levels of mTOR and Redd1 we...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768963/ https://www.ncbi.nlm.nih.gov/pubmed/26846432 http://dx.doi.org/10.3892/mmr.2016.4835 |
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author | CHOI, HEE-SOO AHN, JI HYEON PARK, JOON HA WON, MOO-HO LEE, CHOONG-HYUN |
author_facet | CHOI, HEE-SOO AHN, JI HYEON PARK, JOON HA WON, MOO-HO LEE, CHOONG-HYUN |
author_sort | CHOI, HEE-SOO |
collection | PubMed |
description | Redd1, also known as RTP801/Dig2/DDIT4, is a stress-induced protein and a negative regulator of mammalian target of rapamycin (mTOR). Redd1 is also closely associated with oxidative stress and DNA damage. In the present study, age-related changes in the protein expression levels of mTOR and Redd1 were investigated using immunohistochemistry and western blot in the gerbil hippocampus at postnatal month (PM) 3, 6, 12 and 24. No significant differences were identified in the levels of mTOR among the experimental groups, whereas, the levels of phosphorylated mTOR decreased with age. The protein expression levels of Redd1 were observed to gradually increase with age; in the PM 24 group, the level was significantly increased (~189.2%), compared with the PM 3 group. In addition, Redd1 immunoreactivity was significantly increased in the hippocampal principal neurons of the PM 24 group, including the pyramidal cells in the hippocampus proper and granule cells in the dentate gyrus, compared with the other experimental groups. These results demonstrated that the protein expression of Redd1 in the hippocampus was markedly increased during normal aging, indicating that the age-related increase in the expression of Redd1 may be closely associated with age-related hippocampal change. |
format | Online Article Text |
id | pubmed-4768963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-47689632016-03-08 Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging CHOI, HEE-SOO AHN, JI HYEON PARK, JOON HA WON, MOO-HO LEE, CHOONG-HYUN Mol Med Rep Articles Redd1, also known as RTP801/Dig2/DDIT4, is a stress-induced protein and a negative regulator of mammalian target of rapamycin (mTOR). Redd1 is also closely associated with oxidative stress and DNA damage. In the present study, age-related changes in the protein expression levels of mTOR and Redd1 were investigated using immunohistochemistry and western blot in the gerbil hippocampus at postnatal month (PM) 3, 6, 12 and 24. No significant differences were identified in the levels of mTOR among the experimental groups, whereas, the levels of phosphorylated mTOR decreased with age. The protein expression levels of Redd1 were observed to gradually increase with age; in the PM 24 group, the level was significantly increased (~189.2%), compared with the PM 3 group. In addition, Redd1 immunoreactivity was significantly increased in the hippocampal principal neurons of the PM 24 group, including the pyramidal cells in the hippocampus proper and granule cells in the dentate gyrus, compared with the other experimental groups. These results demonstrated that the protein expression of Redd1 in the hippocampus was markedly increased during normal aging, indicating that the age-related increase in the expression of Redd1 may be closely associated with age-related hippocampal change. D.A. Spandidos 2016-03 2016-01-29 /pmc/articles/PMC4768963/ /pubmed/26846432 http://dx.doi.org/10.3892/mmr.2016.4835 Text en Copyright: © Choi et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles CHOI, HEE-SOO AHN, JI HYEON PARK, JOON HA WON, MOO-HO LEE, CHOONG-HYUN Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title | Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title_full | Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title_fullStr | Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title_full_unstemmed | Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title_short | Age-dependent changes in the protein expression levels of Redd1 and mTOR in the gerbil hippocampus during normal aging |
title_sort | age-dependent changes in the protein expression levels of redd1 and mtor in the gerbil hippocampus during normal aging |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768963/ https://www.ncbi.nlm.nih.gov/pubmed/26846432 http://dx.doi.org/10.3892/mmr.2016.4835 |
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