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The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing

A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-cont...

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Autores principales: Desai, Stuti K, Winardhi, Ricksen S, Periasamy, Saravanan, Dykas, Michal M, Jie, Yan, Kenney, Linda J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769171/
https://www.ncbi.nlm.nih.gov/pubmed/26880544
http://dx.doi.org/10.7554/eLife.10747
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author Desai, Stuti K
Winardhi, Ricksen S
Periasamy, Saravanan
Dykas, Michal M
Jie, Yan
Kenney, Linda J
author_facet Desai, Stuti K
Winardhi, Ricksen S
Periasamy, Saravanan
Dykas, Michal M
Jie, Yan
Kenney, Linda J
author_sort Desai, Stuti K
collection PubMed
description A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles. DOI: http://dx.doi.org/10.7554/eLife.10747.001
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spelling pubmed-47691712016-02-29 The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing Desai, Stuti K Winardhi, Ricksen S Periasamy, Saravanan Dykas, Michal M Jie, Yan Kenney, Linda J eLife Microbiology and Infectious Disease A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles. DOI: http://dx.doi.org/10.7554/eLife.10747.001 eLife Sciences Publications, Ltd 2016-02-02 /pmc/articles/PMC4769171/ /pubmed/26880544 http://dx.doi.org/10.7554/eLife.10747 Text en © 2016, Desai et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Desai, Stuti K
Winardhi, Ricksen S
Periasamy, Saravanan
Dykas, Michal M
Jie, Yan
Kenney, Linda J
The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title_full The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title_fullStr The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title_full_unstemmed The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title_short The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing
title_sort horizontally-acquired response regulator ssrb drives a salmonella lifestyle switch by relieving biofilm silencing
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769171/
https://www.ncbi.nlm.nih.gov/pubmed/26880544
http://dx.doi.org/10.7554/eLife.10747
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