Cargando…

Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus

Two human-infecting avian influenza viruses (AIVs), H7N9 and H10N8, have emerged in China, which further indicate that the H9N2 subtype of AIVs, as an internal gene donor, may have an important role in the generation of new viruses with cross-species transmissibility and pathogenicity. H9N2 viruses...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Ge, Liang, Chai Hong, Hua, Deng Guo, Song, Lei Yong, Xiang, Yang Guo, Guang, Chen, Lan, Chen Hua, Ping, Hua Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770023/
https://www.ncbi.nlm.nih.gov/pubmed/26973600
http://dx.doi.org/10.3389/fmicb.2016.00057
_version_ 1782418180860280832
author Ye, Ge
Liang, Chai Hong
Hua, Deng Guo
Song, Lei Yong
Xiang, Yang Guo
Guang, Chen
Lan, Chen Hua
Ping, Hua Yu
author_facet Ye, Ge
Liang, Chai Hong
Hua, Deng Guo
Song, Lei Yong
Xiang, Yang Guo
Guang, Chen
Lan, Chen Hua
Ping, Hua Yu
author_sort Ye, Ge
collection PubMed
description Two human-infecting avian influenza viruses (AIVs), H7N9 and H10N8, have emerged in China, which further indicate that the H9N2 subtype of AIVs, as an internal gene donor, may have an important role in the generation of new viruses with cross-species transmissibility and pathogenicity. H9N2 viruses that contain such internal genes widely exist in poultry but are rarely reported in migratory birds. In this study, two strains of the H9N2 virus were isolated from fecal samples of migratory birds in 2014: one strain from Caizi Lake in Anhui Province and one from Chen Lake in Hubei Province of China. Nucleotide sequence analysis revealed high homology of all six internal genes of these two strains with the internal genes of the human H10N8 virus in Jiangxi Province, as well as with the human H7N9 virus. Phylogenetic analysis indicated a possible origin of these two strains from poultry in South China. Both of the two viruses tested could replicated in respiratory organs of infective mice without adaption, by both strains of the H9N2 AIVs from wild birds, suggesting their potential capacity for directly infecting mammals. Our findings indicate the existence of H9N2 viruses that contain internal genes highly homologous with human H10N8 or H7N9 viruses. Wild birds can contribute to the spread of the H9N2 virus that contains the “harmful” internal gene complex, leading to gene rearrangement with other influenza viruses and to the generation of new pathogenic viruses. Therefore, strengthening AIV surveillance in wild birds can promote an understanding of the presence and prevalence of viruses and provide scientific evidence for the prevention and control of AIVs and human-infecting AIVs.
format Online
Article
Text
id pubmed-4770023
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-47700232016-03-11 Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus Ye, Ge Liang, Chai Hong Hua, Deng Guo Song, Lei Yong Xiang, Yang Guo Guang, Chen Lan, Chen Hua Ping, Hua Yu Front Microbiol Microbiology Two human-infecting avian influenza viruses (AIVs), H7N9 and H10N8, have emerged in China, which further indicate that the H9N2 subtype of AIVs, as an internal gene donor, may have an important role in the generation of new viruses with cross-species transmissibility and pathogenicity. H9N2 viruses that contain such internal genes widely exist in poultry but are rarely reported in migratory birds. In this study, two strains of the H9N2 virus were isolated from fecal samples of migratory birds in 2014: one strain from Caizi Lake in Anhui Province and one from Chen Lake in Hubei Province of China. Nucleotide sequence analysis revealed high homology of all six internal genes of these two strains with the internal genes of the human H10N8 virus in Jiangxi Province, as well as with the human H7N9 virus. Phylogenetic analysis indicated a possible origin of these two strains from poultry in South China. Both of the two viruses tested could replicated in respiratory organs of infective mice without adaption, by both strains of the H9N2 AIVs from wild birds, suggesting their potential capacity for directly infecting mammals. Our findings indicate the existence of H9N2 viruses that contain internal genes highly homologous with human H10N8 or H7N9 viruses. Wild birds can contribute to the spread of the H9N2 virus that contains the “harmful” internal gene complex, leading to gene rearrangement with other influenza viruses and to the generation of new pathogenic viruses. Therefore, strengthening AIV surveillance in wild birds can promote an understanding of the presence and prevalence of viruses and provide scientific evidence for the prevention and control of AIVs and human-infecting AIVs. Frontiers Media S.A. 2016-02-29 /pmc/articles/PMC4770023/ /pubmed/26973600 http://dx.doi.org/10.3389/fmicb.2016.00057 Text en Copyright © 2016 Ye, Liang, Hua, Song, Xing, Guang, Lan and Ping. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ye, Ge
Liang, Chai Hong
Hua, Deng Guo
Song, Lei Yong
Xiang, Yang Guo
Guang, Chen
Lan, Chen Hua
Ping, Hua Yu
Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title_full Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title_fullStr Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title_full_unstemmed Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title_short Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus
title_sort phylogenetic analysis and pathogenicity assessment of two strains of avian influenza virus subtype h9n2 isolated from migratory birds: high homology of internal genes with human h10n8 virus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770023/
https://www.ncbi.nlm.nih.gov/pubmed/26973600
http://dx.doi.org/10.3389/fmicb.2016.00057
work_keys_str_mv AT yege phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT liangchaihong phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT huadengguo phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT songleiyong phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT xiangyangguo phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT guangchen phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT lanchenhua phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus
AT pinghuayu phylogeneticanalysisandpathogenicityassessmentoftwostrainsofavianinfluenzavirussubtypeh9n2isolatedfrommigratorybirdshighhomologyofinternalgeneswithhumanh10n8virus