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Ethyl pyruvate protects against sepsis by regulating energy metabolism
BACKGROUND: Ethyl pyruvate (EP) is a derivative of pyruvic acid that has been demonstrated to be a potential scavenger of reactive oxygen species as well as an anti-inflammatory agent. In this study, we investigated the protective effects of EP and its role in regulating the energy metabolism in the...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770074/ https://www.ncbi.nlm.nih.gov/pubmed/26966369 http://dx.doi.org/10.2147/TCRM.S97989 |
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author | Kang, Hongjun Mao, Zhi Zhao, Yan Yin, Ting Song, Qing Pan, Liang Hu, Xin Hu, Jie Zhou, Feihu |
author_facet | Kang, Hongjun Mao, Zhi Zhao, Yan Yin, Ting Song, Qing Pan, Liang Hu, Xin Hu, Jie Zhou, Feihu |
author_sort | Kang, Hongjun |
collection | PubMed |
description | BACKGROUND: Ethyl pyruvate (EP) is a derivative of pyruvic acid that has been demonstrated to be a potential scavenger of reactive oxygen species as well as an anti-inflammatory agent. In this study, we investigated the protective effects of EP and its role in regulating the energy metabolism in the livers of cecal-ligation-and-puncture-induced septic mice. METHODS: The animals were treated intraperitoneally with 0.2 mL of Ringer’s lactate solution or an equivalent volume of Ringer’s lactate solution containing EP immediately after cecal ligation and puncture. Each mouse in the Sham group was only subjected to a laparotomy. At 30-, 60-, 180-, and 360-minute time points, we measured the histopathological alterations of the intestines, and the plasma levels of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor-α, and the total antioxidative capacity, malondialdehyde content, and lactate and lactate/pyruvate levels in livers. Furthermore, we detected the levels of adenosine triphosphate, total adenylate, and energy charge in the livers. RESULTS: Our results demonstrated that the administration of EP significantly improved the survival rate and reduced intestinal histological alterations. EP inhibited the plasma levels of IL-1β, IL-6, and tumor necrosis factor-α and increased the IL-10 level. EP significantly inhibited the elevation of the malondialdehyde, lactate, and lactate/pyruvate levels and enhanced the total antioxidative capacity levels in the liver tissues. The downregulation of the adenosine triphosphate, total adenylate, and energy charge levels in the liver tissues was reversed in the septic mice treated with EP. CONCLUSION: The results suggest that EP administration effectively modulates the energy metabolism, which may be an important component in treatment of sepsis. |
format | Online Article Text |
id | pubmed-4770074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47700742016-03-10 Ethyl pyruvate protects against sepsis by regulating energy metabolism Kang, Hongjun Mao, Zhi Zhao, Yan Yin, Ting Song, Qing Pan, Liang Hu, Xin Hu, Jie Zhou, Feihu Ther Clin Risk Manag Original Research BACKGROUND: Ethyl pyruvate (EP) is a derivative of pyruvic acid that has been demonstrated to be a potential scavenger of reactive oxygen species as well as an anti-inflammatory agent. In this study, we investigated the protective effects of EP and its role in regulating the energy metabolism in the livers of cecal-ligation-and-puncture-induced septic mice. METHODS: The animals were treated intraperitoneally with 0.2 mL of Ringer’s lactate solution or an equivalent volume of Ringer’s lactate solution containing EP immediately after cecal ligation and puncture. Each mouse in the Sham group was only subjected to a laparotomy. At 30-, 60-, 180-, and 360-minute time points, we measured the histopathological alterations of the intestines, and the plasma levels of interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor-α, and the total antioxidative capacity, malondialdehyde content, and lactate and lactate/pyruvate levels in livers. Furthermore, we detected the levels of adenosine triphosphate, total adenylate, and energy charge in the livers. RESULTS: Our results demonstrated that the administration of EP significantly improved the survival rate and reduced intestinal histological alterations. EP inhibited the plasma levels of IL-1β, IL-6, and tumor necrosis factor-α and increased the IL-10 level. EP significantly inhibited the elevation of the malondialdehyde, lactate, and lactate/pyruvate levels and enhanced the total antioxidative capacity levels in the liver tissues. The downregulation of the adenosine triphosphate, total adenylate, and energy charge levels in the liver tissues was reversed in the septic mice treated with EP. CONCLUSION: The results suggest that EP administration effectively modulates the energy metabolism, which may be an important component in treatment of sepsis. Dove Medical Press 2016-02-23 /pmc/articles/PMC4770074/ /pubmed/26966369 http://dx.doi.org/10.2147/TCRM.S97989 Text en © 2016 Kang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Kang, Hongjun Mao, Zhi Zhao, Yan Yin, Ting Song, Qing Pan, Liang Hu, Xin Hu, Jie Zhou, Feihu Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title | Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title_full | Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title_fullStr | Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title_full_unstemmed | Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title_short | Ethyl pyruvate protects against sepsis by regulating energy metabolism |
title_sort | ethyl pyruvate protects against sepsis by regulating energy metabolism |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770074/ https://www.ncbi.nlm.nih.gov/pubmed/26966369 http://dx.doi.org/10.2147/TCRM.S97989 |
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