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Myosin VI contributes to malignant proliferation of human glioma cells
Previously characterized as a backward motor, myosin VI (MYO6), which belongs to myosin family, moves toward the minus end of the actin track, a direction opposite to all other known myosin members. Recent researches have illuminated the role of MYO6 in human cancers, particularly in prostate cancer...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770103/ https://www.ncbi.nlm.nih.gov/pubmed/26937209 http://dx.doi.org/10.4196/kjpp.2016.20.2.139 |
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author | Xu, Rong Fang, Xu-hao Zhong, Ping |
author_facet | Xu, Rong Fang, Xu-hao Zhong, Ping |
author_sort | Xu, Rong |
collection | PubMed |
description | Previously characterized as a backward motor, myosin VI (MYO6), which belongs to myosin family, moves toward the minus end of the actin track, a direction opposite to all other known myosin members. Recent researches have illuminated the role of MYO6 in human cancers, particularly in prostate cancer. However, the role of MYO6 in glioma has not yet been determined. In this study, to explore the role of MYO6 in human glioma, lentivirus-delivered short hairpin RNA (shRNA) targeting MYO6 was designed to stably down-regulate its endogenous expression in glioblastoma cells U251. Knockdown of MYO6 signifi cantly inhibited viability and proliferation of U251 cells in vitro. Moreover, the cell cycle of U251 cells was arrested at G0/G1 phase with the absence of MYO6, which could contribute to the suppression of cell proliferation. In conclusion, we firstly identified the crucial involvement of MYO6 in human glioma. The inhibition of MYO6 by shRNA might be a potential therapeutic method in human glioma. |
format | Online Article Text |
id | pubmed-4770103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47701032016-03-02 Myosin VI contributes to malignant proliferation of human glioma cells Xu, Rong Fang, Xu-hao Zhong, Ping Korean J Physiol Pharmacol Original Article Previously characterized as a backward motor, myosin VI (MYO6), which belongs to myosin family, moves toward the minus end of the actin track, a direction opposite to all other known myosin members. Recent researches have illuminated the role of MYO6 in human cancers, particularly in prostate cancer. However, the role of MYO6 in glioma has not yet been determined. In this study, to explore the role of MYO6 in human glioma, lentivirus-delivered short hairpin RNA (shRNA) targeting MYO6 was designed to stably down-regulate its endogenous expression in glioblastoma cells U251. Knockdown of MYO6 signifi cantly inhibited viability and proliferation of U251 cells in vitro. Moreover, the cell cycle of U251 cells was arrested at G0/G1 phase with the absence of MYO6, which could contribute to the suppression of cell proliferation. In conclusion, we firstly identified the crucial involvement of MYO6 in human glioma. The inhibition of MYO6 by shRNA might be a potential therapeutic method in human glioma. The Korean Physiological Society and The Korean Society of Pharmacology 2016-03 2016-02-23 /pmc/articles/PMC4770103/ /pubmed/26937209 http://dx.doi.org/10.4196/kjpp.2016.20.2.139 Text en Copyright © 2016 The Korean Physiological Society and The Korean Society of Pharmacology http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Xu, Rong Fang, Xu-hao Zhong, Ping Myosin VI contributes to malignant proliferation of human glioma cells |
title | Myosin VI contributes to malignant proliferation of human glioma cells |
title_full | Myosin VI contributes to malignant proliferation of human glioma cells |
title_fullStr | Myosin VI contributes to malignant proliferation of human glioma cells |
title_full_unstemmed | Myosin VI contributes to malignant proliferation of human glioma cells |
title_short | Myosin VI contributes to malignant proliferation of human glioma cells |
title_sort | myosin vi contributes to malignant proliferation of human glioma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770103/ https://www.ncbi.nlm.nih.gov/pubmed/26937209 http://dx.doi.org/10.4196/kjpp.2016.20.2.139 |
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