Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis...

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Autores principales: Butkevich, Alexey N., Mitronova, Gyuzel Yu., Sidenstein, Sven C., Klocke, Jessica L., Kamin, Dirk, Meineke, Dirk N. H., D'Este, Elisa, Kraemer, Philip‐Tobias, Danzl, Johann G., Belov, Vladimir N., Hell, Stefan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770443/
https://www.ncbi.nlm.nih.gov/pubmed/26844929
http://dx.doi.org/10.1002/anie.201511018
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author Butkevich, Alexey N.
Mitronova, Gyuzel Yu.
Sidenstein, Sven C.
Klocke, Jessica L.
Kamin, Dirk
Meineke, Dirk N. H.
D'Este, Elisa
Kraemer, Philip‐Tobias
Danzl, Johann G.
Belov, Vladimir N.
Hell, Stefan W.
author_facet Butkevich, Alexey N.
Mitronova, Gyuzel Yu.
Sidenstein, Sven C.
Klocke, Jessica L.
Kamin, Dirk
Meineke, Dirk N. H.
D'Este, Elisa
Kraemer, Philip‐Tobias
Danzl, Johann G.
Belov, Vladimir N.
Hell, Stefan W.
author_sort Butkevich, Alexey N.
collection PubMed
description A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm.
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spelling pubmed-47704432016-04-11 Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells Butkevich, Alexey N. Mitronova, Gyuzel Yu. Sidenstein, Sven C. Klocke, Jessica L. Kamin, Dirk Meineke, Dirk N. H. D'Este, Elisa Kraemer, Philip‐Tobias Danzl, Johann G. Belov, Vladimir N. Hell, Stefan W. Angew Chem Int Ed Engl Communications A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure–property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one‐ and two‐color images of living cells with an optical resolution of 40–60 nm. John Wiley and Sons Inc. 2016-02-04 2016-03-01 /pmc/articles/PMC4770443/ /pubmed/26844929 http://dx.doi.org/10.1002/anie.201511018 Text en © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Communications
Butkevich, Alexey N.
Mitronova, Gyuzel Yu.
Sidenstein, Sven C.
Klocke, Jessica L.
Kamin, Dirk
Meineke, Dirk N. H.
D'Este, Elisa
Kraemer, Philip‐Tobias
Danzl, Johann G.
Belov, Vladimir N.
Hell, Stefan W.
Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title_full Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title_fullStr Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title_full_unstemmed Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title_short Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super‐Resolution STED Microscopy in Living Cells
title_sort fluorescent rhodamines and fluorogenic carbopyronines for super‐resolution sted microscopy in living cells
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770443/
https://www.ncbi.nlm.nih.gov/pubmed/26844929
http://dx.doi.org/10.1002/anie.201511018
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