Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcripti...

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Detalles Bibliográficos
Autores principales: Angermueller, Christof, Clark, Stephen J., Lee, Heather J., Macaulay, Iain C., Teng, Mabel J., Hu, Tim Xiaoming, Krueger, Felix, Smallwood, Sebastien, Ponting, Chris P., Voet, Thierry, Kelsey, Gavin, Stegle, Oliver, Reik, Wolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770512/
https://www.ncbi.nlm.nih.gov/pubmed/26752769
http://dx.doi.org/10.1038/nmeth.3728
Descripción
Sumario:We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method reveals novel associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

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