Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcripti...

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Autores principales: Angermueller, Christof, Clark, Stephen J., Lee, Heather J., Macaulay, Iain C., Teng, Mabel J., Hu, Tim Xiaoming, Krueger, Felix, Smallwood, Sebastien, Ponting, Chris P., Voet, Thierry, Kelsey, Gavin, Stegle, Oliver, Reik, Wolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770512/
https://www.ncbi.nlm.nih.gov/pubmed/26752769
http://dx.doi.org/10.1038/nmeth.3728
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author Angermueller, Christof
Clark, Stephen J.
Lee, Heather J.
Macaulay, Iain C.
Teng, Mabel J.
Hu, Tim Xiaoming
Krueger, Felix
Smallwood, Sebastien
Ponting, Chris P.
Voet, Thierry
Kelsey, Gavin
Stegle, Oliver
Reik, Wolf
author_facet Angermueller, Christof
Clark, Stephen J.
Lee, Heather J.
Macaulay, Iain C.
Teng, Mabel J.
Hu, Tim Xiaoming
Krueger, Felix
Smallwood, Sebastien
Ponting, Chris P.
Voet, Thierry
Kelsey, Gavin
Stegle, Oliver
Reik, Wolf
author_sort Angermueller, Christof
collection PubMed
description We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method reveals novel associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
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spelling pubmed-47705122016-09-01 Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity Angermueller, Christof Clark, Stephen J. Lee, Heather J. Macaulay, Iain C. Teng, Mabel J. Hu, Tim Xiaoming Krueger, Felix Smallwood, Sebastien Ponting, Chris P. Voet, Thierry Kelsey, Gavin Stegle, Oliver Reik, Wolf Nat Methods Article We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method reveals novel associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes. 2016-01-11 2016-03 /pmc/articles/PMC4770512/ /pubmed/26752769 http://dx.doi.org/10.1038/nmeth.3728 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Angermueller, Christof
Clark, Stephen J.
Lee, Heather J.
Macaulay, Iain C.
Teng, Mabel J.
Hu, Tim Xiaoming
Krueger, Felix
Smallwood, Sebastien
Ponting, Chris P.
Voet, Thierry
Kelsey, Gavin
Stegle, Oliver
Reik, Wolf
Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title_full Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title_fullStr Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title_full_unstemmed Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title_short Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
title_sort parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770512/
https://www.ncbi.nlm.nih.gov/pubmed/26752769
http://dx.doi.org/10.1038/nmeth.3728
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