Purification, Visualization, and Molecular Signature of Neural Stem Cells

Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1as a novel...

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Autores principales: Yu, Yuan Hong, Narayanan, Gunaseelan, Sankaran, Shvetha, Ramasamy, Srinivas, Chan, Shi Yu, Lin, Shuping, Chen, Jinmiao, Yang, Henry, Srivats, Hariharan, Ahmed, Sohail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2016
Materias:
Original Research Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770853/
https://www.ncbi.nlm.nih.gov/pubmed/26464067
http://dx.doi.org/10.1089/scd.2015.0190
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author Yu, Yuan Hong
Narayanan, Gunaseelan
Sankaran, Shvetha
Ramasamy, Srinivas
Chan, Shi Yu
Lin, Shuping
Chen, Jinmiao
Yang, Henry
Srivats, Hariharan
Ahmed, Sohail
author_facet Yu, Yuan Hong
Narayanan, Gunaseelan
Sankaran, Shvetha
Ramasamy, Srinivas
Chan, Shi Yu
Lin, Shuping
Chen, Jinmiao
Yang, Henry
Srivats, Hariharan
Ahmed, Sohail
author_sort Yu, Yuan Hong
collection PubMed
description Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1as a novel marker for NSCs and show that it can be used along with Lewis-X (LeX) to yield a highly purified population of NSCs. Using time-lapse microscopy, we are able to follow NSCs forming neurospheres, allowing their visualization. Finally, using single-cell polymerase chain reaction (PCR), we determine the molecular signature of NSCs. The single-cell PCR data suggest that along with the Notch and Shh pathways, the Hippo pathway plays an important role in NSC activity.
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spelling pubmed-47708532016-03-17 Purification, Visualization, and Molecular Signature of Neural Stem Cells Yu, Yuan Hong Narayanan, Gunaseelan Sankaran, Shvetha Ramasamy, Srinivas Chan, Shi Yu Lin, Shuping Chen, Jinmiao Yang, Henry Srivats, Hariharan Ahmed, Sohail Stem Cells Dev Original Research Reports Neural stem cells (NSCs) are isolated from primary brain tissue and propagated as a heterogeneous mix of cells, including neural progenitors. To date, NSCs have not been purified in vitro to allow study of their biology and utility in regenerative medicine. In this study, we identify C1qR1as a novel marker for NSCs and show that it can be used along with Lewis-X (LeX) to yield a highly purified population of NSCs. Using time-lapse microscopy, we are able to follow NSCs forming neurospheres, allowing their visualization. Finally, using single-cell polymerase chain reaction (PCR), we determine the molecular signature of NSCs. The single-cell PCR data suggest that along with the Notch and Shh pathways, the Hippo pathway plays an important role in NSC activity. Mary Ann Liebert, Inc. 2016-01-15 2015-10-14 /pmc/articles/PMC4770853/ /pubmed/26464067 http://dx.doi.org/10.1089/scd.2015.0190 Text en © Yuan Hong Yu, et al., 2016; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (<http://creativecommons.org/licenses/by-nc/4.0/>) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Research Reports
Yu, Yuan Hong
Narayanan, Gunaseelan
Sankaran, Shvetha
Ramasamy, Srinivas
Chan, Shi Yu
Lin, Shuping
Chen, Jinmiao
Yang, Henry
Srivats, Hariharan
Ahmed, Sohail
Purification, Visualization, and Molecular Signature of Neural Stem Cells
title Purification, Visualization, and Molecular Signature of Neural Stem Cells
title_full Purification, Visualization, and Molecular Signature of Neural Stem Cells
title_fullStr Purification, Visualization, and Molecular Signature of Neural Stem Cells
title_full_unstemmed Purification, Visualization, and Molecular Signature of Neural Stem Cells
title_short Purification, Visualization, and Molecular Signature of Neural Stem Cells
title_sort purification, visualization, and molecular signature of neural stem cells
topic Original Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4770853/
https://www.ncbi.nlm.nih.gov/pubmed/26464067
http://dx.doi.org/10.1089/scd.2015.0190
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