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Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation

Organohalides are recalcitrant pollutants that have been responsible for substantial contamination of soils and groundwater. Organohalide-respiring bacteria (ORB) provide a potential solution to remediate contaminated sites, through their ability to use organohalides as terminal electron acceptors t...

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Autores principales: Jugder, Bat-Erdene, Ertan, Haluk, Bohl, Susanne, Lee, Matthew, Marquis, Christopher P., Manefield, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771760/
https://www.ncbi.nlm.nih.gov/pubmed/26973626
http://dx.doi.org/10.3389/fmicb.2016.00249
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author Jugder, Bat-Erdene
Ertan, Haluk
Bohl, Susanne
Lee, Matthew
Marquis, Christopher P.
Manefield, Michael
author_facet Jugder, Bat-Erdene
Ertan, Haluk
Bohl, Susanne
Lee, Matthew
Marquis, Christopher P.
Manefield, Michael
author_sort Jugder, Bat-Erdene
collection PubMed
description Organohalides are recalcitrant pollutants that have been responsible for substantial contamination of soils and groundwater. Organohalide-respiring bacteria (ORB) provide a potential solution to remediate contaminated sites, through their ability to use organohalides as terminal electron acceptors to yield energy for growth (i.e., organohalide respiration). Ideally, this process results in non- or lesser-halogenated compounds that are mostly less toxic to the environment or more easily degraded. At the heart of these processes are reductive dehalogenases (RDases), which are membrane bound enzymes coupled with other components that facilitate dehalogenation of organohalides to generate cellular energy. This review focuses on RDases, concentrating on those which have been purified (partially or wholly) and functionally characterized. Further, the paper reviews the major bacteria involved in organohalide breakdown and the evidence for microbial evolution of RDases. Finally, the capacity for using ORB in a bioremediation and bioaugmentation capacity are discussed.
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spelling pubmed-47717602016-03-11 Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation Jugder, Bat-Erdene Ertan, Haluk Bohl, Susanne Lee, Matthew Marquis, Christopher P. Manefield, Michael Front Microbiol Microbiology Organohalides are recalcitrant pollutants that have been responsible for substantial contamination of soils and groundwater. Organohalide-respiring bacteria (ORB) provide a potential solution to remediate contaminated sites, through their ability to use organohalides as terminal electron acceptors to yield energy for growth (i.e., organohalide respiration). Ideally, this process results in non- or lesser-halogenated compounds that are mostly less toxic to the environment or more easily degraded. At the heart of these processes are reductive dehalogenases (RDases), which are membrane bound enzymes coupled with other components that facilitate dehalogenation of organohalides to generate cellular energy. This review focuses on RDases, concentrating on those which have been purified (partially or wholly) and functionally characterized. Further, the paper reviews the major bacteria involved in organohalide breakdown and the evidence for microbial evolution of RDases. Finally, the capacity for using ORB in a bioremediation and bioaugmentation capacity are discussed. Frontiers Media S.A. 2016-03-01 /pmc/articles/PMC4771760/ /pubmed/26973626 http://dx.doi.org/10.3389/fmicb.2016.00249 Text en Copyright © 2016 Jugder, Ertan, Bohl, Lee, Marquis and Manefield. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Jugder, Bat-Erdene
Ertan, Haluk
Bohl, Susanne
Lee, Matthew
Marquis, Christopher P.
Manefield, Michael
Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title_full Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title_fullStr Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title_full_unstemmed Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title_short Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation
title_sort organohalide respiring bacteria and reductive dehalogenases: key tools in organohalide bioremediation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771760/
https://www.ncbi.nlm.nih.gov/pubmed/26973626
http://dx.doi.org/10.3389/fmicb.2016.00249
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