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Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies

Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. He...

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Autor principal: Chandrashekar, Vani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771897/
https://www.ncbi.nlm.nih.gov/pubmed/26989559
http://dx.doi.org/10.1155/2016/2698362
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author Chandrashekar, Vani
author_facet Chandrashekar, Vani
author_sort Chandrashekar, Vani
collection PubMed
description Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman's rank correlation was used to study correlation between A1c levels and age. Mann-Whitney U test was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders.
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spelling pubmed-47718972016-03-17 Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies Chandrashekar, Vani Scientifica (Cairo) Research Article Hb A1c measurement is subject to interference by hemoglobin traits and this is dependent on the method used for determination. In this paper we studied the difference between Hb A1c measured by HPLC in hemoglobin traits and normal chromatograms. We also studied the correlation of Hb A1c with age. Hemoglobin analysis was carried out by high performance liquid chromatography. Spearman's rank correlation was used to study correlation between A1c levels and age. Mann-Whitney U test was used to study the difference in Hb A1c between patients with normal hemoglobin and hemoglobin traits. A total of 431 patients were studied. There was positive correlation with age in patients with normal chromatograms only. No correlation was seen in Hb E trait or beta thalassemia trait. No significant difference in Hb A1c of patients with normal chromatograms and patients with hemoglobin traits was seen. There is no interference by abnormal hemoglobin in the detection of A1c by high performance liquid chromatography. This method cannot be used for detection of A1c in compound heterozygous and homozygous disorders. Hindawi Publishing Corporation 2016 2016-02-16 /pmc/articles/PMC4771897/ /pubmed/26989559 http://dx.doi.org/10.1155/2016/2698362 Text en Copyright © 2016 Vani Chandrashekar. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chandrashekar, Vani
Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title_full Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title_fullStr Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title_full_unstemmed Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title_short Hb A1c Separation by High Performance Liquid Chromatography in Hemoglobinopathies
title_sort hb a1c separation by high performance liquid chromatography in hemoglobinopathies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771897/
https://www.ncbi.nlm.nih.gov/pubmed/26989559
http://dx.doi.org/10.1155/2016/2698362
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