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Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery

We studied the age-dependent regulation of the expression of the antioxidant enzyme manganese superoxide dismutase (MnSOD encoded by Sod2) through promoter methylation. C57Bl/6 mice were either (i) sedentary (SED), (ii) treated with the antioxidant catechin (CAT), or (iii) voluntarily exercised (EX)...

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Autores principales: Nguyen, Albert, Leblond, François, Mamarbachi, Maya, Geoffroy, Steve, Thorin, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771915/
https://www.ncbi.nlm.nih.gov/pubmed/26989455
http://dx.doi.org/10.1155/2016/8627384
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author Nguyen, Albert
Leblond, François
Mamarbachi, Maya
Geoffroy, Steve
Thorin, Eric
author_facet Nguyen, Albert
Leblond, François
Mamarbachi, Maya
Geoffroy, Steve
Thorin, Eric
author_sort Nguyen, Albert
collection PubMed
description We studied the age-dependent regulation of the expression of the antioxidant enzyme manganese superoxide dismutase (MnSOD encoded by Sod2) through promoter methylation. C57Bl/6 mice were either (i) sedentary (SED), (ii) treated with the antioxidant catechin (CAT), or (iii) voluntarily exercised (EX) from weaning (1-month old; mo) to 9 mo. Then, all mice aged sedentarily and were untreated until 12 mo. Sod2 promoter methylation was similar in all groups in 9 mo but decreased (p < 0.05) in 12 mo SED mice only, which was associated with an increased (p < 0.05) transcriptional activity in vitro. At all ages, femoral artery endothelial function was maintained; this was due to an increased (p < 0.05) contribution of eNOS-derived NO in 12 mo SED mice only. CAT and EX prevented these changes in age-related endothelial function. Thus, a ROS-dependent epigenetic positive regulation of Sod2 gene expression likely represents a defense mechanism prolonging eNOS function in aging mouse femoral arteries.
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spelling pubmed-47719152016-03-17 Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery Nguyen, Albert Leblond, François Mamarbachi, Maya Geoffroy, Steve Thorin, Eric Oxid Med Cell Longev Research Article We studied the age-dependent regulation of the expression of the antioxidant enzyme manganese superoxide dismutase (MnSOD encoded by Sod2) through promoter methylation. C57Bl/6 mice were either (i) sedentary (SED), (ii) treated with the antioxidant catechin (CAT), or (iii) voluntarily exercised (EX) from weaning (1-month old; mo) to 9 mo. Then, all mice aged sedentarily and were untreated until 12 mo. Sod2 promoter methylation was similar in all groups in 9 mo but decreased (p < 0.05) in 12 mo SED mice only, which was associated with an increased (p < 0.05) transcriptional activity in vitro. At all ages, femoral artery endothelial function was maintained; this was due to an increased (p < 0.05) contribution of eNOS-derived NO in 12 mo SED mice only. CAT and EX prevented these changes in age-related endothelial function. Thus, a ROS-dependent epigenetic positive regulation of Sod2 gene expression likely represents a defense mechanism prolonging eNOS function in aging mouse femoral arteries. Hindawi Publishing Corporation 2016 2016-02-16 /pmc/articles/PMC4771915/ /pubmed/26989455 http://dx.doi.org/10.1155/2016/8627384 Text en Copyright © 2016 Albert Nguyen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nguyen, Albert
Leblond, François
Mamarbachi, Maya
Geoffroy, Steve
Thorin, Eric
Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title_full Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title_fullStr Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title_full_unstemmed Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title_short Age-Dependent Demethylation of Sod2 Promoter in the Mouse Femoral Artery
title_sort age-dependent demethylation of sod2 promoter in the mouse femoral artery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771915/
https://www.ncbi.nlm.nih.gov/pubmed/26989455
http://dx.doi.org/10.1155/2016/8627384
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