Cargando…

Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics

The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty art...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Bin, Huang, Huizhu, Li, Zhong, Gong, Mengjuan, Shi, Wan, Zhu, Chunxia, Gu, Zulian, Zou, Zhongjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771918/
https://www.ncbi.nlm.nih.gov/pubmed/26989428
http://dx.doi.org/10.1155/2016/9398435
_version_ 1782418469811126272
author Han, Bin
Huang, Huizhu
Li, Zhong
Gong, Mengjuan
Shi, Wan
Zhu, Chunxia
Gu, Zulian
Zou, Zhongjie
author_facet Han, Bin
Huang, Huizhu
Li, Zhong
Gong, Mengjuan
Shi, Wan
Zhu, Chunxia
Gu, Zulian
Zou, Zhongjie
author_sort Han, Bin
collection PubMed
description The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty arthritis could significantly attenuate the ankle joint swelling, and this beneficial antigout effect might be mediated, at least in part, by inhibiting tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) production in synovial fluid as well as nuclear transcription factor-κB p65 (NF-κB p65) protein expression in synovial tissue. Moreover, metabonomic analysis demonstrated that 5 and 6 potential biomarkers associated with gouty arthritis in plasma and urine, respectively, which were mainly involved in energy metabolism, amino acid metabolism, and gut microbe metabolism, were identified. HG could reverse the pathological process of MSU-induced gouty arthritis through regulating the disturbed metabolic pathways. These results provided important mechanistic insights into the protective effects of HG against MSU-induced gouty arthritis in rats.
format Online
Article
Text
id pubmed-4771918
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-47719182016-03-17 Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics Han, Bin Huang, Huizhu Li, Zhong Gong, Mengjuan Shi, Wan Zhu, Chunxia Gu, Zulian Zou, Zhongjie Evid Based Complement Alternat Med Research Article The present study was undertaken to evaluate the therapeutic effects of Huzhang-Guizhi herb pair (HG), firstly included in Hu-Zhang Power documented in Taiping Shenghui Fang, on monosodium urate (MSU) crystals-induced gouty arthritis in rats. We found that pretreatment with HG in rats with gouty arthritis could significantly attenuate the ankle joint swelling, and this beneficial antigout effect might be mediated, at least in part, by inhibiting tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) production in synovial fluid as well as nuclear transcription factor-κB p65 (NF-κB p65) protein expression in synovial tissue. Moreover, metabonomic analysis demonstrated that 5 and 6 potential biomarkers associated with gouty arthritis in plasma and urine, respectively, which were mainly involved in energy metabolism, amino acid metabolism, and gut microbe metabolism, were identified. HG could reverse the pathological process of MSU-induced gouty arthritis through regulating the disturbed metabolic pathways. These results provided important mechanistic insights into the protective effects of HG against MSU-induced gouty arthritis in rats. Hindawi Publishing Corporation 2016 2016-02-16 /pmc/articles/PMC4771918/ /pubmed/26989428 http://dx.doi.org/10.1155/2016/9398435 Text en Copyright © 2016 Bin Han et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Han, Bin
Huang, Huizhu
Li, Zhong
Gong, Mengjuan
Shi, Wan
Zhu, Chunxia
Gu, Zulian
Zou, Zhongjie
Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title_full Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title_fullStr Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title_full_unstemmed Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title_short Therapeutic Effects of Chinese Medicine Herb Pair, Huzhang and Guizhi, on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats Revealed by Anti-Inflammatory Assessments and NMR-Based Metabonomics
title_sort therapeutic effects of chinese medicine herb pair, huzhang and guizhi, on monosodium urate crystal-induced gouty arthritis in rats revealed by anti-inflammatory assessments and nmr-based metabonomics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771918/
https://www.ncbi.nlm.nih.gov/pubmed/26989428
http://dx.doi.org/10.1155/2016/9398435
work_keys_str_mv AT hanbin therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT huanghuizhu therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT lizhong therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT gongmengjuan therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT shiwan therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT zhuchunxia therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT guzulian therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics
AT zouzhongjie therapeuticeffectsofchinesemedicineherbpairhuzhangandguizhionmonosodiumuratecrystalinducedgoutyarthritisinratsrevealedbyantiinflammatoryassessmentsandnmrbasedmetabonomics