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Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation
RATIONALE: Telomerase is a nuclear regulator of telomere elongation with recent reports suggesting a role in regulation of mitochondrial reactive oxygen species. Flow-mediated dilation in patients with cardiovascular disease is dependent on the formation of reactive oxygen species. OBJECTIVE: We exa...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772813/ https://www.ncbi.nlm.nih.gov/pubmed/26699654 http://dx.doi.org/10.1161/CIRCRESAHA.115.307918 |
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author | Beyer, Andreas M. Freed, Julie K. Durand, Matthew J. Riedel, Michael Ait-Aissa, Karima Green, Paula Hockenberry, Joseph C. Morgan, R. Garret Donato, Anthony J. Peleg, Refael Gasparri, Mario Rokkas, Chris K. Santos, Janine H. Priel, Esther Gutterman, David D. |
author_facet | Beyer, Andreas M. Freed, Julie K. Durand, Matthew J. Riedel, Michael Ait-Aissa, Karima Green, Paula Hockenberry, Joseph C. Morgan, R. Garret Donato, Anthony J. Peleg, Refael Gasparri, Mario Rokkas, Chris K. Santos, Janine H. Priel, Esther Gutterman, David D. |
author_sort | Beyer, Andreas M. |
collection | PubMed |
description | RATIONALE: Telomerase is a nuclear regulator of telomere elongation with recent reports suggesting a role in regulation of mitochondrial reactive oxygen species. Flow-mediated dilation in patients with cardiovascular disease is dependent on the formation of reactive oxygen species. OBJECTIVE: We examined the hypothesis that telomerase activity modulates microvascular flow-mediated dilation, and loss of telomerase activity contributes to the change of mediator from nitric oxide to mitochondrial hydrogen peroxide in patients with coronary artery disease (CAD). METHODS AND RESULTS: Human coronary and adipose arterioles were isolated for videomicroscopy. Flow-mediated dilation was measured in vessels pretreated with the telomerase inhibitor BIBR-1532 or vehicle. Statistical differences between groups were determined using a 2-way analysis of variance repeated measure (n≥4; P<0.05). L-NAME (N(ω)-nitro-L-arginine methyl ester; nitric oxide synthase inhibitor) abolished flow-mediated dilation in arterioles from subjects without CAD, whereas polyethylene glycol-catalase (PEG-catalase; hydrogen peroxide scavenger) had no effect. After exposure to BIBR-1532, arterioles from non-CAD subjects maintained the magnitude of dilation but changed the mediator from nitric oxide to mitochondrial hydrogen peroxide (% max diameter at 100 cm H(2)O: vehicle 74.6±4.1, L-NAME 37.0±2.0*, PEG-catalase 82.1±2.8; BIBR-1532 69.9±4.0, L-NAME 84.7±2.2, PEG-catalase 36.5±6.9*). Conversely, treatment of microvessels from CAD patients with the telomerase activator AGS 499 converted the PEG-catalase-inhibitable dilation to one mediated by nitric oxide (% max diameter at 100 cm H(2)O: adipose, AGS 499 78.5±3.9; L-NAME 10.9±17.5*; PEG-catalase 79.2±4.9). Endothelial-independent dilation was not altered with either treatment. CONCLUSIONS: We have identified a novel role for telomerase in re-establishing a physiological mechanism of vasodilation in arterioles from subjects with CAD. These findings suggest a new target for reducing the oxidative milieu in the microvasculature of patients with CAD. |
format | Online Article Text |
id | pubmed-4772813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-47728132016-03-19 Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation Beyer, Andreas M. Freed, Julie K. Durand, Matthew J. Riedel, Michael Ait-Aissa, Karima Green, Paula Hockenberry, Joseph C. Morgan, R. Garret Donato, Anthony J. Peleg, Refael Gasparri, Mario Rokkas, Chris K. Santos, Janine H. Priel, Esther Gutterman, David D. Circ Res Clinical Track RATIONALE: Telomerase is a nuclear regulator of telomere elongation with recent reports suggesting a role in regulation of mitochondrial reactive oxygen species. Flow-mediated dilation in patients with cardiovascular disease is dependent on the formation of reactive oxygen species. OBJECTIVE: We examined the hypothesis that telomerase activity modulates microvascular flow-mediated dilation, and loss of telomerase activity contributes to the change of mediator from nitric oxide to mitochondrial hydrogen peroxide in patients with coronary artery disease (CAD). METHODS AND RESULTS: Human coronary and adipose arterioles were isolated for videomicroscopy. Flow-mediated dilation was measured in vessels pretreated with the telomerase inhibitor BIBR-1532 or vehicle. Statistical differences between groups were determined using a 2-way analysis of variance repeated measure (n≥4; P<0.05). L-NAME (N(ω)-nitro-L-arginine methyl ester; nitric oxide synthase inhibitor) abolished flow-mediated dilation in arterioles from subjects without CAD, whereas polyethylene glycol-catalase (PEG-catalase; hydrogen peroxide scavenger) had no effect. After exposure to BIBR-1532, arterioles from non-CAD subjects maintained the magnitude of dilation but changed the mediator from nitric oxide to mitochondrial hydrogen peroxide (% max diameter at 100 cm H(2)O: vehicle 74.6±4.1, L-NAME 37.0±2.0*, PEG-catalase 82.1±2.8; BIBR-1532 69.9±4.0, L-NAME 84.7±2.2, PEG-catalase 36.5±6.9*). Conversely, treatment of microvessels from CAD patients with the telomerase activator AGS 499 converted the PEG-catalase-inhibitable dilation to one mediated by nitric oxide (% max diameter at 100 cm H(2)O: adipose, AGS 499 78.5±3.9; L-NAME 10.9±17.5*; PEG-catalase 79.2±4.9). Endothelial-independent dilation was not altered with either treatment. CONCLUSIONS: We have identified a novel role for telomerase in re-establishing a physiological mechanism of vasodilation in arterioles from subjects with CAD. These findings suggest a new target for reducing the oxidative milieu in the microvasculature of patients with CAD. Lippincott Williams & Wilkins 2016-03-04 2016-03-03 /pmc/articles/PMC4772813/ /pubmed/26699654 http://dx.doi.org/10.1161/CIRCRESAHA.115.307918 Text en © 2015 The Authors. Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDervis (https://creativecommons.org/licenses/by-nc-nd/3.0/) License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made. |
spellingShingle | Clinical Track Beyer, Andreas M. Freed, Julie K. Durand, Matthew J. Riedel, Michael Ait-Aissa, Karima Green, Paula Hockenberry, Joseph C. Morgan, R. Garret Donato, Anthony J. Peleg, Refael Gasparri, Mario Rokkas, Chris K. Santos, Janine H. Priel, Esther Gutterman, David D. Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title | Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title_full | Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title_fullStr | Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title_full_unstemmed | Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title_short | Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation |
title_sort | critical role for telomerase in the mechanism of flow-mediated dilation in the human microcirculation |
topic | Clinical Track |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772813/ https://www.ncbi.nlm.nih.gov/pubmed/26699654 http://dx.doi.org/10.1161/CIRCRESAHA.115.307918 |
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