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Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing

BACKGROUND: Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes invol...

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Detalles Bibliográficos
Autores principales: Jin, Hangxia, Dong, Dekun, Yang, Qinghua, Zhu, Danhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773115/
https://www.ncbi.nlm.nih.gov/pubmed/26930632
http://dx.doi.org/10.1371/journal.pone.0150504
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author Jin, Hangxia
Dong, Dekun
Yang, Qinghua
Zhu, Danhua
author_facet Jin, Hangxia
Dong, Dekun
Yang, Qinghua
Zhu, Danhua
author_sort Jin, Hangxia
collection PubMed
description BACKGROUND: Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte. RESULTS: Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance. CONCLUSIONS: This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted.
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spelling pubmed-47731152016-03-07 Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing Jin, Hangxia Dong, Dekun Yang, Qinghua Zhu, Danhua PLoS One Research Article BACKGROUND: Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte. RESULTS: Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance. CONCLUSIONS: This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted. Public Library of Science 2016-03-01 /pmc/articles/PMC4773115/ /pubmed/26930632 http://dx.doi.org/10.1371/journal.pone.0150504 Text en © 2016 Jin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jin, Hangxia
Dong, Dekun
Yang, Qinghua
Zhu, Danhua
Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title_full Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title_fullStr Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title_full_unstemmed Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title_short Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing
title_sort salt-responsive transcriptome profiling of suaeda glauca via rna sequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773115/
https://www.ncbi.nlm.nih.gov/pubmed/26930632
http://dx.doi.org/10.1371/journal.pone.0150504
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