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MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value

Moringa oleifera is a widespread plant with substantial nutritional and medicinal value. We postulated that microRNAs (miRNAs), which are endogenous, noncoding small RNAs regulating gene expression at the post-transcriptional level, might contribute to the medicinal properties of plants of this spec...

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Autores principales: Pirrò, Stefano, Zanella, Letizia, Kenzo, Maurice, Montesano, Carla, Minutolo, Antonella, Potestà, Marina, Sobze, Martin Sanou, Canini, Antonella, Cirilli, Marco, Muleo, Rosario, Colizzi, Vittorio, Galgani, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773123/
https://www.ncbi.nlm.nih.gov/pubmed/26930203
http://dx.doi.org/10.1371/journal.pone.0149495
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author Pirrò, Stefano
Zanella, Letizia
Kenzo, Maurice
Montesano, Carla
Minutolo, Antonella
Potestà, Marina
Sobze, Martin Sanou
Canini, Antonella
Cirilli, Marco
Muleo, Rosario
Colizzi, Vittorio
Galgani, Andrea
author_facet Pirrò, Stefano
Zanella, Letizia
Kenzo, Maurice
Montesano, Carla
Minutolo, Antonella
Potestà, Marina
Sobze, Martin Sanou
Canini, Antonella
Cirilli, Marco
Muleo, Rosario
Colizzi, Vittorio
Galgani, Andrea
author_sort Pirrò, Stefano
collection PubMed
description Moringa oleifera is a widespread plant with substantial nutritional and medicinal value. We postulated that microRNAs (miRNAs), which are endogenous, noncoding small RNAs regulating gene expression at the post-transcriptional level, might contribute to the medicinal properties of plants of this species after ingestion into human body, regulating human gene expression. However, the knowledge is scarce about miRNA in Moringa. Furthermore, in order to test the hypothesis on the pharmacological potential properties of miRNA, we conducted a high-throughput sequencing analysis using the Illumina platform. A total of 31,290,964 raw reads were produced from a library of small RNA isolated from M. oleifera seeds. We identified 94 conserved and two novel miRNAs that were validated by qRT-PCR assays. Results from qRT-PCR trials conducted on the expression of 20 Moringa miRNA showed that are conserved across multiple plant species as determined by their detection in tissue of other common crop plants. In silico analyses predicted target genes for the conserved miRNA that in turn allowed to relate the miRNAs to the regulation of physiological processes. Some of the predicted plant miRNAs have functional homology to their mammalian counterparts and regulated human genes when they were transfected into cell lines. To our knowledge, this is the first report of discovering M. oleifera miRNAs based on high-throughput sequencing and bioinformatics analysis and we provided new insight into a potential cross-species control of human gene expression. The widespread cultivation and consumption of M. oleifera, for nutritional and medicinal purposes, brings humans into close contact with products and extracts of this plant species. The potential for miRNA transfer should be evaluated as one possible mechanism of action to account for beneficial properties of this valuable species.
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spelling pubmed-47731232016-03-07 MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value Pirrò, Stefano Zanella, Letizia Kenzo, Maurice Montesano, Carla Minutolo, Antonella Potestà, Marina Sobze, Martin Sanou Canini, Antonella Cirilli, Marco Muleo, Rosario Colizzi, Vittorio Galgani, Andrea PLoS One Research Article Moringa oleifera is a widespread plant with substantial nutritional and medicinal value. We postulated that microRNAs (miRNAs), which are endogenous, noncoding small RNAs regulating gene expression at the post-transcriptional level, might contribute to the medicinal properties of plants of this species after ingestion into human body, regulating human gene expression. However, the knowledge is scarce about miRNA in Moringa. Furthermore, in order to test the hypothesis on the pharmacological potential properties of miRNA, we conducted a high-throughput sequencing analysis using the Illumina platform. A total of 31,290,964 raw reads were produced from a library of small RNA isolated from M. oleifera seeds. We identified 94 conserved and two novel miRNAs that were validated by qRT-PCR assays. Results from qRT-PCR trials conducted on the expression of 20 Moringa miRNA showed that are conserved across multiple plant species as determined by their detection in tissue of other common crop plants. In silico analyses predicted target genes for the conserved miRNA that in turn allowed to relate the miRNAs to the regulation of physiological processes. Some of the predicted plant miRNAs have functional homology to their mammalian counterparts and regulated human genes when they were transfected into cell lines. To our knowledge, this is the first report of discovering M. oleifera miRNAs based on high-throughput sequencing and bioinformatics analysis and we provided new insight into a potential cross-species control of human gene expression. The widespread cultivation and consumption of M. oleifera, for nutritional and medicinal purposes, brings humans into close contact with products and extracts of this plant species. The potential for miRNA transfer should be evaluated as one possible mechanism of action to account for beneficial properties of this valuable species. Public Library of Science 2016-03-01 /pmc/articles/PMC4773123/ /pubmed/26930203 http://dx.doi.org/10.1371/journal.pone.0149495 Text en © 2016 Pirrò et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pirrò, Stefano
Zanella, Letizia
Kenzo, Maurice
Montesano, Carla
Minutolo, Antonella
Potestà, Marina
Sobze, Martin Sanou
Canini, Antonella
Cirilli, Marco
Muleo, Rosario
Colizzi, Vittorio
Galgani, Andrea
MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title_full MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title_fullStr MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title_full_unstemmed MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title_short MicroRNA from Moringa oleifera: Identification by High Throughput Sequencing and Their Potential Contribution to Plant Medicinal Value
title_sort microrna from moringa oleifera: identification by high throughput sequencing and their potential contribution to plant medicinal value
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773123/
https://www.ncbi.nlm.nih.gov/pubmed/26930203
http://dx.doi.org/10.1371/journal.pone.0149495
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