Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis

The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported th...

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Autores principales: Takemoto, Noriaki, Yoshimura, Takuji, Miyazaki, Satsuki, Tashiro, Fumi, Miyazaki, Jun-ichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773171/
https://www.ncbi.nlm.nih.gov/pubmed/26930067
http://dx.doi.org/10.1371/journal.pone.0150390
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author Takemoto, Noriaki
Yoshimura, Takuji
Miyazaki, Satsuki
Tashiro, Fumi
Miyazaki, Jun-ichi
author_facet Takemoto, Noriaki
Yoshimura, Takuji
Miyazaki, Satsuki
Tashiro, Fumi
Miyazaki, Jun-ichi
author_sort Takemoto, Noriaki
collection PubMed
description The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice.
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spelling pubmed-47731712016-03-07 Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis Takemoto, Noriaki Yoshimura, Takuji Miyazaki, Satsuki Tashiro, Fumi Miyazaki, Jun-ichi PLoS One Research Article The unknown protein family 0224 (UPF0224) includes three members that are expressed in germ-line cells in mice: Gtsf1, Gtsf1l, and BC048502 (Gtsf2). These genes produce proteins with two repeats of the CHHC Zn-finger domain, a predicted RNA-binding motif, in the N terminus. We previously reported that Gtsf1 is essential for spermatogenesis and retrotransposon suppression. In this study, we investigated the expression patterns and functions of Gtsf1l and Gtsf2. Interestingly, Gtsf1l and Gtsf2 were found to be sequentially but not simultaneously expressed in gonocytes and spermatids. Pull-down experiments showed that both GTSF1L and GTSF2 can interact with PIWI-protein complexes. Nevertheless, knocking out Gtsf1, Gtsf2, or both did not cause defects in spermatogenesis or retrotransposon suppression in mice. Public Library of Science 2016-03-01 /pmc/articles/PMC4773171/ /pubmed/26930067 http://dx.doi.org/10.1371/journal.pone.0150390 Text en © 2016 Takemoto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Takemoto, Noriaki
Yoshimura, Takuji
Miyazaki, Satsuki
Tashiro, Fumi
Miyazaki, Jun-ichi
Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title_full Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title_fullStr Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title_full_unstemmed Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title_short Gtsf1l and Gtsf2 Are Specifically Expressed in Gonocytes and Spermatids but Are Not Essential for Spermatogenesis
title_sort gtsf1l and gtsf2 are specifically expressed in gonocytes and spermatids but are not essential for spermatogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773171/
https://www.ncbi.nlm.nih.gov/pubmed/26930067
http://dx.doi.org/10.1371/journal.pone.0150390
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