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Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha

Mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (RGM) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. Therefore, the id...

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Autores principales: das Neves, Rogerio Coutinho, Trentini, Monalisa Martins, de Castro e Silva, Juliana, Simon, Karina Smidt, Bocca, Anamelia Lorenzetti, Silva, Luciano Paulino, Mortari, Marcia Renata, Kipnis, Andre, Junqueira-Kipnis, Ana Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773228/
https://www.ncbi.nlm.nih.gov/pubmed/26930596
http://dx.doi.org/10.1371/journal.pone.0149729
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author das Neves, Rogerio Coutinho
Trentini, Monalisa Martins
de Castro e Silva, Juliana
Simon, Karina Smidt
Bocca, Anamelia Lorenzetti
Silva, Luciano Paulino
Mortari, Marcia Renata
Kipnis, Andre
Junqueira-Kipnis, Ana Paula
author_facet das Neves, Rogerio Coutinho
Trentini, Monalisa Martins
de Castro e Silva, Juliana
Simon, Karina Smidt
Bocca, Anamelia Lorenzetti
Silva, Luciano Paulino
Mortari, Marcia Renata
Kipnis, Andre
Junqueira-Kipnis, Ana Paula
author_sort das Neves, Rogerio Coutinho
collection PubMed
description Mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (RGM) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. Therefore, the identification of new drugs to improve the current treatment or lower the risk of inducing resistance is urgently needed. Wasp venom primarily comprises peptides that are responsible for most of the biological activities in this poison. Here, a novel peptide Polydim-I, from Polybia dimorpha Neotropical wasp, was explored as an antimycobacterial agent. Polydim-I provoked cell wall disruption and exhibited non-cytotoxicity towards mammalian cells. Polydim-I treatment of macrophages infected with different M. abscessus subsp. massiliense strains reduced 40 to 50% of the bacterial load. Additionally, the Polydim-I treatment of highly susceptible mice intravenously infected with M. abscessus subsp. massiliense induced 0.8 to 1 log reduction of the bacterial load in the lungs, spleen, and liver. In conclusion, this is the first study to show the therapeutic potential of a peptide derived from wasp venom in treating mycobacteria infections. Polydim-I acts on the M. abscessus subsp. massiliense cell wall and reduce 40–90% of the bacterial load both in vitro and in vivo. The presented results encourage further studies on the use of Polydim-I as one of the components for M. abscessus subsp. massiliense treatment.
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spelling pubmed-47732282016-03-07 Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha das Neves, Rogerio Coutinho Trentini, Monalisa Martins de Castro e Silva, Juliana Simon, Karina Smidt Bocca, Anamelia Lorenzetti Silva, Luciano Paulino Mortari, Marcia Renata Kipnis, Andre Junqueira-Kipnis, Ana Paula PLoS One Research Article Mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (RGM) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. Therefore, the identification of new drugs to improve the current treatment or lower the risk of inducing resistance is urgently needed. Wasp venom primarily comprises peptides that are responsible for most of the biological activities in this poison. Here, a novel peptide Polydim-I, from Polybia dimorpha Neotropical wasp, was explored as an antimycobacterial agent. Polydim-I provoked cell wall disruption and exhibited non-cytotoxicity towards mammalian cells. Polydim-I treatment of macrophages infected with different M. abscessus subsp. massiliense strains reduced 40 to 50% of the bacterial load. Additionally, the Polydim-I treatment of highly susceptible mice intravenously infected with M. abscessus subsp. massiliense induced 0.8 to 1 log reduction of the bacterial load in the lungs, spleen, and liver. In conclusion, this is the first study to show the therapeutic potential of a peptide derived from wasp venom in treating mycobacteria infections. Polydim-I acts on the M. abscessus subsp. massiliense cell wall and reduce 40–90% of the bacterial load both in vitro and in vivo. The presented results encourage further studies on the use of Polydim-I as one of the components for M. abscessus subsp. massiliense treatment. Public Library of Science 2016-03-01 /pmc/articles/PMC4773228/ /pubmed/26930596 http://dx.doi.org/10.1371/journal.pone.0149729 Text en © 2016 das Neves et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
das Neves, Rogerio Coutinho
Trentini, Monalisa Martins
de Castro e Silva, Juliana
Simon, Karina Smidt
Bocca, Anamelia Lorenzetti
Silva, Luciano Paulino
Mortari, Marcia Renata
Kipnis, Andre
Junqueira-Kipnis, Ana Paula
Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title_full Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title_fullStr Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title_full_unstemmed Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title_short Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha
title_sort antimycobacterial activity of a new peptide polydim-i isolated from neotropical social wasp polybia dimorpha
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773228/
https://www.ncbi.nlm.nih.gov/pubmed/26930596
http://dx.doi.org/10.1371/journal.pone.0149729
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