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Rapid Synthesis of a Long Double-Stranded Oligonucleotide from a Single-Stranded Nucleotide Using Magnetic Beads and an Oligo Library
Chemical synthesis of oligonucleotides is a widely used tool in the field of biochemistry. Several methods for gene synthesis have been introduced in the growing area of genomics. In this paper, a novel method of constructing dsDNA is proposed. Short (28-mer) oligo fragments from a library were asse...
Autores principales: | Pengpumkiat, Sumate, Koesdjojo, Myra, Rowley, Erik R., Mockler, Todd C., Remcho, Vincent T. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773231/ https://www.ncbi.nlm.nih.gov/pubmed/26930667 http://dx.doi.org/10.1371/journal.pone.0149774 |
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