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Membrane Blebbing Is Required for Mesenchymal Precursor Migration
Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773234/ https://www.ncbi.nlm.nih.gov/pubmed/26930466 http://dx.doi.org/10.1371/journal.pone.0150004 |
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author | de Lucas, Beatriz Bernal, Aurora M. Pérez, Laura San Martín, Nuria Gálvez, Beatriz G. |
author_facet | de Lucas, Beatriz Bernal, Aurora M. Pérez, Laura San Martín, Nuria Gálvez, Beatriz G. |
author_sort | de Lucas, Beatriz |
collection | PubMed |
description | Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols. |
format | Online Article Text |
id | pubmed-4773234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47732342016-03-07 Membrane Blebbing Is Required for Mesenchymal Precursor Migration de Lucas, Beatriz Bernal, Aurora M. Pérez, Laura San Martín, Nuria Gálvez, Beatriz G. PLoS One Research Article Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols. Public Library of Science 2016-03-01 /pmc/articles/PMC4773234/ /pubmed/26930466 http://dx.doi.org/10.1371/journal.pone.0150004 Text en © 2016 de Lucas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article de Lucas, Beatriz Bernal, Aurora M. Pérez, Laura San Martín, Nuria Gálvez, Beatriz G. Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title | Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title_full | Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title_fullStr | Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title_full_unstemmed | Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title_short | Membrane Blebbing Is Required for Mesenchymal Precursor Migration |
title_sort | membrane blebbing is required for mesenchymal precursor migration |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773234/ https://www.ncbi.nlm.nih.gov/pubmed/26930466 http://dx.doi.org/10.1371/journal.pone.0150004 |
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