Cargando…

Membrane Blebbing Is Required for Mesenchymal Precursor Migration

Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is...

Descripción completa

Detalles Bibliográficos
Autores principales: de Lucas, Beatriz, Bernal, Aurora, M. Pérez, Laura, San Martín, Nuria, Gálvez, Beatriz G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773234/
https://www.ncbi.nlm.nih.gov/pubmed/26930466
http://dx.doi.org/10.1371/journal.pone.0150004
_version_ 1782418700614238208
author de Lucas, Beatriz
Bernal, Aurora
M. Pérez, Laura
San Martín, Nuria
Gálvez, Beatriz G.
author_facet de Lucas, Beatriz
Bernal, Aurora
M. Pérez, Laura
San Martín, Nuria
Gálvez, Beatriz G.
author_sort de Lucas, Beatriz
collection PubMed
description Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols.
format Online
Article
Text
id pubmed-4773234
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47732342016-03-07 Membrane Blebbing Is Required for Mesenchymal Precursor Migration de Lucas, Beatriz Bernal, Aurora M. Pérez, Laura San Martín, Nuria Gálvez, Beatriz G. PLoS One Research Article Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols. Public Library of Science 2016-03-01 /pmc/articles/PMC4773234/ /pubmed/26930466 http://dx.doi.org/10.1371/journal.pone.0150004 Text en © 2016 de Lucas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Lucas, Beatriz
Bernal, Aurora
M. Pérez, Laura
San Martín, Nuria
Gálvez, Beatriz G.
Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title_full Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title_fullStr Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title_full_unstemmed Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title_short Membrane Blebbing Is Required for Mesenchymal Precursor Migration
title_sort membrane blebbing is required for mesenchymal precursor migration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4773234/
https://www.ncbi.nlm.nih.gov/pubmed/26930466
http://dx.doi.org/10.1371/journal.pone.0150004
work_keys_str_mv AT delucasbeatriz membraneblebbingisrequiredformesenchymalprecursormigration
AT bernalaurora membraneblebbingisrequiredformesenchymalprecursormigration
AT mperezlaura membraneblebbingisrequiredformesenchymalprecursormigration
AT sanmartinnuria membraneblebbingisrequiredformesenchymalprecursormigration
AT galvezbeatrizg membraneblebbingisrequiredformesenchymalprecursormigration